瓶囊碘泡虫HSP70基因的克隆、鉴定及功能预测

Cloning,Identification and Functional Prediction of Heat Shock Protein 70 Gene in Myxobolus ampullicapsulatus

HSP70(Heat shock proteins 70)是分子量约为70kD的热休克蛋白,具有保护细胞和生命体的重要作用。通过分子克隆和染色体步移技术首次获得瓶囊碘泡虫(Myxobolus ampullicapsulatus Zhao et al.,2008)HSP70家族同源基因的完整序列,并利用生物信息学方法对该基因进行鉴定,同时对它编码的蛋白序列进行分析。结果显示:瓶囊碘泡虫HSP70蛋白为HSPA8同源物,命名为MaHSPA8;MaHSPA8基因核苷酸序列全长2 696bp,共编码662个氨基酸,MaHSPA8蛋白相对分子量为72.5kD,等电点为5.37,无跨膜区和信号肽,共有24个潜在的抗原位点。此发现将为进一步研究HSP70基因在瓶囊碘泡虫寄生过程中的作用提供基础资料。

HSP70s is group of heat shock proteins with molecular weight about 70 kD and play an important role during protecting cells or organism from being poisoned. In this study, one HSP70 family member＇s complete sequence of Myxobolus ampullicapsu- latus was firstly cloned and analyzed by using bioinformatic approaches and genome walking technique so as to acquire its basic infor- mation, and bioinformatic methods were used to identify the gene and analysis its protein sequence. Results show that M. ampulli- capsulatus HSP70 is the homology o[ HSPA8, named as MaHSPA8. MaHSPA8, the full length of which is 2 696 bp, encodes 662 amino acids with predicted molecular weight 72.5 kD, pI 5.3. Additionally, 24 potential antigenic sites were predicted for this pro tein, but it has no transmembrane region as well as signal peptide. All of these results would provide the foundational data for in depth research of the HSP70＇s role during the parasitic process of M. ampullicapsulatus in the fishes.