地尔硫卓对血管紧张素Ⅱ损伤内皮细胞的保护作用研究

Protective effects of diltiazem on damaged vascular endothelial cells induced by angiotensin Ⅱ

目的观察地尔硫卓对血管紧张素Ⅱ损伤的人脐静脉内皮细胞的保护作用。方法体外培养人脐静脉内皮细胞,用1×10^(-6)mol·L^(-1)的血管紧张素Ⅱ作为损伤因子模拟体内损伤模型。用3-(4,5-二甲基噻唑-2)-2(MTT)法检测细胞活力,用流式细胞术检测细胞凋亡率,对细胞上清液乳酸脱氢酶活力进行检测。观察地尔硫卓对血管紧张素Ⅱ损伤的人脐静脉内皮细胞的保护作用。结果与对照组相比,血管紧张素Ⅱ(1×10^(-7),1×10^(-6),1×10-5mol·L^(-1))呈浓度依赖性及时间依赖性降低细胞活力和细胞凋亡率。地尔硫卓能够呈浓度依赖性提高细胞活力和凋亡率,且与血管紧张素Ⅱ相比,地尔硫卓能够呈浓度依赖性的减少细胞上清液乳酸脱氢酶活力。结论地尔硫卓对血管紧张素Ⅱ损伤的人脐静脉内皮细胞具有保护作用。

Objective To investigate the protective effects of diltiazem（ Dil） on damaged human umbilical vein endothelial cells induced by angiotensin Ⅱ（ Ang-Ⅱ）. Methods Human umbilical vein endothelial cells（ HUVECs） were cultured in vitro,with 1 × 10^-6mol·L^-1Ang-Ⅱas an injury factor simulating the injury model. The cell viability was assessed using MTT metabolism assay. The apoptosis rate of HUVECs was detected by flow cytometry, The production of lactate dehydrogenase（ LDH） activity in cell supernatant was detected by colorimetry. The protective effect of diltiazemon damaged HUVECs induced by Ang-Ⅱ was investigated. Results Compared with control group,Ang-Ⅱ（ 1 × 10^-7,1 × 10^-6,1 × 10^（-5）mol · L^-1） concentration-and time-dependently decrease the cell vitality and the apoptosis rate of HUVECs. Pretreatment with dilconcentration-dependently improved the cell vitality and the apoptosis rate of HUVECs. In addition,compared with the damaged group,diltiazem reduced LDH activity in the cell supernatant. Conclusion Diltiazem has protective effect on the damaged HUVECs induced by Ang-Ⅱ.