芦荟多糖对实验性结肠炎大鼠肠黏膜的保护作用及其机制研究

The protection effect and mechanism of aloe polysaccharide on experimental colitis mucosa

目的:探讨芦荟多糖对实验性结肠炎大鼠肠黏膜的保护作用。方法:成功建立2,4,6-三硝基苯磺酸(TNBS)实验性大鼠结肠炎模型,给予芦荟多糖治疗,并与柳氮磺吡啶肠溶片(SASP)做对照。结果:模型组大鼠DAI评分和组织病理学评分均高于正常组(P<0.01),而各给药组经治疗后各项指标均降低(P<0.01)。模型组IL-12和IFN-γ mRNA和蛋白表达水平高于正常组(P<0.01),而各给药组IL-12和IFN-γ表达水平低于模型组(P<0.01)。模型组IL-4和IL-13 mRNA和蛋白表达水平低于正常组(P<0.01),而各给药组IL-4和IL-13 mRNA表达水平高于模型组(P<0.01)。结论:芦荟多糖对实验性结肠炎大鼠结肠黏膜有良好的保护作用,作用机制可能通过调节肠黏膜局部免疫平衡来发挥作用的。

Objective To investigate the protective effect of aloe polysaccharide on experimental colitis mucosal. Methods The TNBS-indueed experimental rat colitis model was successfully established and were treated by aloe polysaccharide, and enteric-coated tablets with sulfasalazine （SASP）. Results DAI score, gross morphology score, histopathologic score and MPO in the model group were significantly higher than that in the normal group （P 〈 0.01 ）, while those indicators were significantly lower after treatment （P 〈 0.01 ）, IL-12 and IFN-γ mRNA and protein expressions in the model group were significantly higher than that in the normal group （P 〈 0.01 ）, and IL-12 and IFN-γ expression levels in treatment group were significantly lower than that in the model group （P 〈 0.01）, IL-4 and IL-13 mRNA and protein expression levels in the model group were significantly lower than that in the normal group （P 〈 0.01 ）, and IL-4 and IL-13 expression in treatment group was significantly higher than that in the model group （P 〈 0.01）. Conclusion Aloe polysaceharide on experimental colitis in rats has a good protective effect, of which mechanism may be reduced by IFN-γ and IL- 12 levels, increased IL-4, IL-13 levels, and correct the imbalance of Th1/Th2.