bFGF基因转染BMSCs对COPD大鼠肺部炎症因子表达的影响

The influence of bFGF gene transfected BMSCs on inflammatory cytokines expression of COPD rat

目的:研究碱性成纤维因子(bFGF)基因转染骨髓间充质干细胞(BMSCs)对慢性阻塞性肺疾病(COPD)大鼠肺组织炎症因子表达的影响。方法 :分离、培养SD大鼠BMSCs;脂质体转染法导入bFGF基因;造模后分6组:正常对照组(NC组)、COPD组(A组)、BMSCs组(B组)、pc DNA3.1-BMSCs组(C组)、bFGFpc DNA3.1 BMSCs组(D组)、bFGF组(E组);QRT-PCR法检测各组肺组织TNF-α、IL-1β基因表达。结果 :与A组相比,B组、C组、D组两种炎症因子基因的表达显著下降(P<0.05),而B组、C组、D组之间比较无显著差异(P>0.05)。结论:外源性bFGF-pc DNA3.1-BMSCs、pcDNA3.1-BMSCs、BMSCs移植对COPD大鼠肺组织中TNF-α、IL-1β的表达均有较为明显的抑制作用,但是bFGF-pcDNA3.1-BMSCs的抑制炎症因子作用效果并不优于单纯的BMSCs。

Objective To study the influence of bFGF gene transfected bone marrow-derived mesenchymal stem cells （BMSCs） on the inflammatory cytokines of COPD rat. Methods The BMSCs were separated from SD rat and cultured and then bFGF gene was imported to BMSCs by liposome transfection method. The samples were prepared into six groups： normal control group, COPD group （A）, BMSCs group （B）, pcDNA3.1-BMSCs group （C）, bFGF-pcDNA3.1-BMSCs group （D）, and bFGF group （E）. The expressions of TNF-α and IL-1β by QRT- PCR were detected. Results Compared with COPD group, TNF-α and IL-1β genes from groups B to D dropped significantly （P 〈 0.05）. The changes of TNF-α and IL-1β genes among groups B to D showed no significant difference （P 〉 0.05）. Conclusion BFGF transfected BMSCs, sample BMSCs and pcDNA3.1 transfected BMSCs can inhibit the expression of inflammatory cytokines of TNF-α and IL-1β, but there is no obvious advantage in comparison to bFGF transfected BMSCs and sample BMSCs in respect of inhibiting the expression of inflammatory cytokines of TNF-α and IL-1β.