腹腔感染性脓毒症可溶性髓样细胞触发受体（sTREM-1）与Janus激酶-信号转导和转录激活因子（JAK／STAT）通路的关系

The correlation between the soluble triggering receptor expressed on myeloid cells-1 and the janus kinase/signal transducer and activator of transcription （JAK/STAT） pathway in rats with abdominal sepsis

目的通过动物脓毒症模型探讨可溶性髓样细胞触发受体（sTREM-1）与Janus激酶-信号转导和转录激活因子（JAK／STAT）通路的关系。方法采用盲肠结扎穿孔（CLP）法制作大鼠脓毒症模型，大鼠随机（随机数字法）分为正常对照组（n=6）。假手术组（n=24）、CLP组（n=48）、JAK2抑制剂（AG490）组（n=48）和STAT3抑制剂（雷帕霉素，RPM）组（n=48）。留取外周血行流式细胞仪分析CD4＋CD25＋Treg细胞／CD4＋T细胞比值.采厢逆转录多聚酶链式反应（RT-PCR）测定肝组织sTREM-1mRNA表达水平。结果CLP后6h肝sTREM-1mRNA表达即高于对照组和假手术组，且随时间变化逐渐升高。AG490组肝组织sTREM-1mRNA的表达任术后6h、24h（1．572±0．051，2．063±0．025）较同时间点CLP组（1．592±0．036，2．082±0．021）差异无统计学意义（P〉0．05），而在48h和72hAG490组肝组织sTREM-1mRNA的表达（2．522±0．083，3．153±0．021）低于同时间点CLP组（2．592±0．055，3．204±0．013）（P〈0．05）。术后6hRPM组肝组织sTREM-1mRNA的表达（1．581±0．017）较CLP组差异无统计学意义（P〉0．05），而在术后24、48、72hRPM组肝组织sTREM-1mRNA的表达（1．486±0．019，1．263±0．011，1．115±0．022）显著低于同时间点CLP组肝组织sTREM-1mRNA的表达（P〈0．05）。结论sTREM-1因子与JAK／STAT通路有关，阻断JAK／STAT通路能够抑制sTREM-1mRNA的表达，减缓脓毒症炎症反应的进展。

Objective To investigate the correlation between the soluble triggering receptor expressed on myeloid cells-1 （sTREM-1） and the janus kinase/signal transducer and activator of transcription （JAK/STAT） pathway in rats with abdominal sepsis. Methods Using a sepsis model produced by cecal ligation and puncture （CLP） , Wistar rats were randomly （random number） divided into normal control group （n = 6）, sham-operated group （ n = 24, ） CLP group （ n = 48 ） , AG490 （JAK2 inhibitor） treatment group （ n = 48） and rapamycin （RPM , STAT3 inhibitor） treatment group （n = 48）. At different intervals, the rats in each group were sacrificed, then the peripheral blood cells were harvested to detect the percent of CD4 ＋ CD25 ＋ Treg cells in CD4 ＋ cells by flow cytometry. Meanwhile, mRNA of sTREM-1 from the tissue samples of the liver was also measured by real-time reverse transcription-polymerase chain reaction （RT-PCR）. Results Colnpared withthe normal control group and the sham-operated group, expression of sTREM-1 in the CLP group was significantly higher, and it showed a gradually increased tendency over time. At 6 and 24 hours, there were no significant differences in the expressions of sTREM-1 between the AG490 group （ 1. 57± 0. 051, 2. 063 ±0. 025, respectively） and the CLP group （1. 592 ±0. 036, 2. 082±0. 021, respectively）. But the expression of sTREM-lin the AG490 group （2. 522 ± 0. 083, 3. 153 ±0. 021, respectively） was lower than those in the CLP group （2. 592 ±0. 055, 3. 204 ±0. 013, respectively） during 48 and 72 hours. In addition, there was no significant difference in the expression of sTREM-I between the RPM group （1. 581 ±0.017） and the CLP group （ 1. 592±0. 036） at 6 hours. And the expression of sTREM-lin the RPM group （1. 486 ±0. 019, 1. 263±0. 011, 1.115 ±0. 022, respectively） was significantly lower than those in the CLP group （2. 082 ±0. 021, 2. 592 ± 0. 055, 3. 204± 0. 013, respectively） during 24 to 72 hours. Conclusions These data proved that blocking JAK/STAT pathway could inhibit the expression of sTREM-1 and decelarate the progress of inflammatory reaction in rats with abdominal sepsis.