缩泉丸中益智仁盐炙前后对肾阳虚大鼠肾脏功能的改善作用

Improvement of Renal Function of Adenine-induced Kidney-yang Deficiency Rats Before and After Being Processed of Alpiniae Oxyphyllae Fructus in Suoquan Wan

目的:通过分析缩泉丸中益智仁盐炙前后对肾阳虚多尿模型大鼠肾脏功能的改善作用及肾脏水通道蛋白2(AQP-2)表达的影响,探讨"盐炙入肾-肾主水"的作用机制。方法:采用腺嘌呤致肾阳虚多尿大鼠模型,比较缩泉丸中益智仁盐炙前后对肾阳虚多尿模型大鼠脏器系数、尿量,血中肌酐、尿素氮和β2-微球蛋白,肾脏AQP-2 mRNA和蛋白表达差异的影响。结果:与模型组相比,给药组能降低大鼠尿量,对各脏器指数的变化有一定抑制作用,降低血清肌酐、尿素氮和β2-微球蛋白含量的异常升高;提高AQP-2 mRNA和蛋白的表达,且与生品组相比,盐炙品组降低尿素氮,β2-微球蛋白含量和提高AQP-2mRNA和蛋白的表达作用显著增强。结论:益智仁盐炙后纳入缩泉丸中能较好地改善模型大鼠的肾脏功能和AQP-2的表达,盐炙后作用明显增强,证实了缩泉丸中益智仁盐炙增强疗效的科学性。

Objective： To discuss mechanism of stir fried with salt into the kidney-the kidney controlling water metabolism by analyzing effects of being processed of Alpiniae Oxyphyllae Fructus in Suoquan Wan on renal function of adenine-induced kidney-yang deficiency rats with much urine and expression of aquaporin-2（AQP-2）. Method： Effects of being processed of Alpiniae Oxyphyllae Fructus in Suoquan Wan on urine volume,viscera indexes,creatinine （Cr）,urea nitrogen （BUN） and β2-microglobulin （β2-MG） in blood,AQP-2 mRNA and protein expression of adenine-induced kidney-yang deficiency rats were investigated. Result： Compared with the model group,each treatment group could reduce urine of rats,change of viscera indexes could be obviously inhibit,contents of Cr,BUN and β2-MG in blood significantly reduced,expression of AQP-2 mRNA and protein increased.After being processed with salt of Alpiniae Oxyphyllae Fructus in Suoquan Wan,effects strengthened obviously in comparison with the raw group. Conclusion： Alpiniae Oxyphyllae Fructus before and after being processed in Suoquan Wan have improving effects on renal function and protein expression of AQP-2 of adenine-induced kidney-yang deficiency rats,but effects strengthen obviously after salt processing.