白藜芦醇对MGC803细胞凋亡因子Bad,p-Bad,Caspase-3的影响

Effect of Resveratrol on Expression of Bad,p-Bad and Caspase-3 Protein in MGC803 Cells

目的:探讨白藜芦醇(resveratrol,Res)对人胃癌细胞MGC803细胞凋亡因子及其磷酸化的影响,研究白藜芦醇抗胃癌的作用机制。方法:采用0,50,100,200μmol·L^(-1)Res处理MGC803细胞后,采用台盼蓝法测定MGC803细胞生长抑制率;流式细胞术(FCM)检测细胞凋亡率;免疫组化法检测促凋亡蛋白(Bad),含半胱氨酸的天冬氨酸蛋白水解酶-3(Caspase-3)蛋白表达;蛋白质印迹法(Western blot)检测Bad,磷酸化Bcl-x1/Bcl-2相关死亡启动子(p-Bad),Caspase-3蛋白的表达。结果:随着浓度增加Res抑制胃癌MGC803细胞生长作用明显增强(P<0.01);Res(100μmol·L^(-1))能明显下调MGC803细胞中Bad,p-Bad蛋白表达,同时可上调Caspase-3蛋白表达。结论:Res可以诱导MGC803细胞凋亡,其凋亡与浓度、时间有一定的依赖性;Res诱导MGC803细胞凋亡可能与其下调Bad,p-Bad蛋白和上调Caspase-3蛋白表达有关。

Objective： To observe the effects of resveratrol （Res） on MGC803 cells apoptosis and study its mechanism of inhibiting gastric cancer. Method： After MGC803 cells were treated with 0, 50, 100, 200 μmol · L^-1 Res, inhibition rate of MGC803 cells growth was detected by trypan blue assay. Apoptosis rate was detected by flow cytometry （FCM）. Expressions of Bad and Caspase-3 protein in MGC803 cells were measured by immunohistochemistry method. Expressions of Bad, p-Bad, Caspase-3 protein were detected by Western blot. Result： Res inhibited the growth of MGC803 cells in time-and concentration-dependent manner （P〈0.01）. Res （100 μmol · L^-1） can significantly down-regulate the protein expressions of Bad, p-Bad and up-regulate the protein expression of Caspase-3 in MGC803 cells. Conclusion： Res can induce apoptosis of MGC803 cells in time-and concentration-dependent manner, and the mechanism may be associated with down-regulating protein expressions of Bad, p-Bad and up-regulating protein expressions of Caspase-3.