马蔺子素对大鼠放射性肺损伤的防护作用

Irisquinone Protects Against Radiation-induced Lung Injury in Rats

目的:探讨马蔺子素对SD大鼠放射性肺损伤的防护作用。方法:将210只SD大鼠随机分为0.9%氯化钠溶液组(正常组),马蔺子素组(单药组),单纯照射组(模型组),激素组,马蔺子素大、中、小剂量(60,30,15 mg·kg^(-1)·d^(-1))组,每组30只。采用^(60)Coγ单次20 Gy右全肺照射,建立大鼠放射性肺损伤模型。照射后第1,4,8,12,24周取大鼠肺组织苏木素伊红(HE)染色,马松三色(Masson)染色进行组织病理学观察;紫外分光光度计检测肺组织中羟脯氨酸含量;酶联免疫吸附测定法(ELISA)检测血清肿瘤坏死因子-α(TNF-α),白细胞介素-1β(IL^(-1)β)和转化生长因子-β_1(TGF-β_1)浓度;免疫组化法检测肺组织中TNF-α,TGF-β_1的表达。结果:与模型组比较,大、中剂量马蔺子素照射组可显著降低放射性肺损伤的程度,且有降低羟脯氨酸含量和抑制TNF-α,IL^(-1)β,TGF-β_1表达的作用(P<0.05)。单药组各项指标与正常组相当。结论:中、大剂量马蔺子素对放射性肺损伤有较好的防护作用,且对大鼠肺组织几乎无毒性。

Objective： To investigate the protective effect of irisquinone for radiation-inducedlung injury （RILI） in SD rats. Method： Two hundred and ten SD rats were randomly divided into 0.9% sodium chloride solution group （normal group）,irisquinone group （single drug group）, radiation group （model group）, hormone group, high-dose （60 mg · kg^-1·d^-1, medium-dose （30 mg · kg^-1·d^-1, and low-dose （15 mg · kg^-1·d^-1 irisquinone＋radiation group, n=30 in each group. The models of RILI in SD rats were established by irradiating right whole chest with a single dose of 20 Gy using 60Co γ therapeutic machine. Rats were sacrificed from the seven groups at week 1, 4, 8, 12 and 24 post-irradiation, and the pathological changes of the lung were observed by hematoxylin-eosin（HE） staining and Masson staining; content of hydroxyproline in lung tissues was detected by UV spectrophotometer, tumor necrosis factor-α（TNF-α）, interleukin-1β（IL-1β） and transforming growth factor β1（TGF-β1） in serum were detected by enzyme-linked immunosorbent assay, expression of TNF-α and TGF-β1 in lung tissues were detected by immunohistochemical method. Result： High-dose and medium-dose irisquinone＋radiation group could significantly ameliorate radiation-inducedlung injury, effectively reduce the content of hydroxyproline and inhibite the expression of TNF-α, IL-1β and TGF-β1, with statistically significant difference compared with model group （P〈0.05）. There was no significant difference between the low-dose irisquinone＋radiation group and the model group. There was no statistically significant difference between the single drug group and the normal group. Conclusion： The high-dose and medium-dose irisquinone has protective effect on the radiation-induced lung injury and is nearly non-toxic for the lung tissues of rats.