室间隔缺损相关的长链非编码RNA TUC40-的生物信息学及表达谱分析

Bioinformatic and Expression Analysis of Ventricular Septal Defect-associated Long Non-coding RNA TUC40-

目的探讨TUC40-在人和小鼠心脏胚胎发育过程中是否发挥作用。方法应用生物信息学网站NCBI、UCSC、Uniprot及软件Clustal、DNAMAN、MEGA 6等,对TUC40-及其编码片段uc.40-进行分析;采用链特异性逆转录实时定量聚合酶链反应检测TUC40-与其正义链产物Pbx1 mRNA在小鼠胚胎心脏发育关键时间点的表达谱。结果 Uc.40-于核苷酸序列、基因组位置及转录因子结合位点3个水平在人和小鼠具有保守性。TUC40-与Pbx1 mRNA在小鼠心脏胚胎发育过程中呈现负性趋势。结论 Uc.40-的多水平保守性提示了TUC40-功能的保守性。TUC40-可能是通过调控Pbx1,在人和小鼠的心脏胚胎发育中发挥作用。

Objective To explore the potential role of TUC40- in human and mouse embryonic heart development. Methods Bioinformatics databases including NCBI,UCSC,and Uniprot and software including Clustal,DNAMAN,and MEGA 6 were used to collect information of TUC40- and uc. 40-. The expression profile at key time points of heart development was investigated by strand-specific quantitative real time polymerase chain reaction. Results Uc. 40- was conservative in sequence,genomic location,and transcription factor binding sites across human and mouse. Pbx1 / TUC40- showed negative trend during embryonic mouse heart maturation. Conclusions Various levels of conservation of uc. 40- suggests similar functions of TUC40- in these twospecies. TUC40- may play its roles in human and mouse embryonic heart development by regulating Pbx1.