银杏内酯B对心肌缺血再灌注损伤保护作用的实验研究

Study of Protective Effects of Ginkgolide B on Myocardial Ischemia Reperfusion Injury in Rats

目的观察银杏内酯B对心肌缺血再灌注(MI/R)损伤的保护作用,探讨其作用机制。方法采用冠状动脉结扎使心肌缺血30min再灌注2h的方法建立MI/R损伤大鼠模型,按随机数字表法分为模型组、银杏内酯B三个剂量组(2、4、8mg/kg)、假手术组(n=10),于术前1h和再灌注即刻分别进行尾静脉注射给药。取血清,测定CK、LDH、SOD活性及MDA含量;取心脏,测定心肌梗死面积。结果银杏内酯B高剂量可明显缩小MI/R损伤大鼠的心肌梗死面积,与模型组比较差异有统计学意义(P<0.05);银杏内酯B高、中剂量可显著降低血清CK和LDH活性(P<0.05或P<0.01),低剂量可明显降低血清CK活力(P<0.05);银杏内酯B各剂量组的血清SOD活性显著升高(P<0.05或P<0.01),血清MDA含量则明显降低(P<0.01)。结论银杏内酯B预处理对MI/R损伤的保护作用与其减少自由基的产生、抑制脂质过氧化反应等有关。

Objective To investigate the protective effects of ginkgolide B（ GB） on myocardial ischemia reperfusion injury in rats. Methods Rat model was made by coronary artery ligation for 30 mins followed by 2-hour reperfusion. Then rats were randomly divided into 5 groups（ n = 10）,namely sham,model,GB high,medium and low dose groups（ 8,4 and 2 mg / kg,respectively）,the groups were injected via tail vein twice at 1h pre-operation and the start of the reperfusion,respectively.The CK,LDH and SOD activities and MDA contents in sera were examined,and the area of myocardial infarction was calculated after the reperfusion. Results The area of myocardial infarction was significantly reduced in GB high dose group（ 8 mg /kg）,compared to that of model group（ P 〈 0. 05）. The CK and LDH activities in GB high and medium dose groups were significantly lower than those of model group（ P 〈 0. 05）. GB evidently elevated the SOD activity（ P 〈 0. 05）,and the MDA contents was reduced in sera（ P 〈 0. 01）. Conclusions The effectiveness of GB pretreatment in myocardial ischemia reperfusion injury appears to be associated with the reduction of production of free radical and inhibition of lipid peroxidation.