西瑞香素对肺癌A549细胞侵袭及迁移能力的影响

Effects of daphnoretin on invasion and migration of A549 lung cancer cells

目的探讨西瑞香素对肺癌A549细胞侵袭及迁移能力的影响。方法通过Transwell小室分析西瑞香素对肺癌A549细胞侵袭能力的影响;划痕实验检测西瑞香素对肺癌A549细胞迁移能力的影响;Western blotting检测西瑞香素作用于A549细胞后MMP-2、MMP-9侵袭相关蛋白的表达。结果 Transwell小室实验结果提示,西瑞香素对A549细胞侵袭能力有抑制作用;划痕实验结果提示,西瑞香素对A549细胞迁移能力有抑制作用;Western blotting结果提示,西瑞香素抑制MMP-2、MMP-9侵袭相关蛋白的表达。随着药物浓度增加,MMP-2、MMP-9表达减少,具有剂量依赖性。结论西瑞香素对A549细胞体外侵袭及迁移有抑制作用,这种作用与抑制MMP-2、MMP-9表达有关。

Objective To investigate the effect of daphnoretin on the proliferation and invasion of A549 lung cancer cells,Methods The effects of daphnoretin on the invasion of lung cancer A549 cells was detected by transwell assays and the effects of daphnoretin on the metastasis of lung cancer A549 cells was detected by Wound Healing.The expression of MMP-2,MMP-9 was evaluated by Western blotting on A549 with daphnoretin.Results Daphnoretin was effective in inhibiting the invasion and migration of A549 cells,Western blotting analysis revealed that daphnoretin decreased the protein levels of MMP-2,MMP-9 in a dose-dependent manner.Conclusion Daphnoretin inhibited the invasion and metastasis of lung cancer cell line A549 by regulating the expression of MMP-2 and MMP-9.