Smac/DIABLO对胰腺癌SW1990细胞凋亡及化疗敏感性的影响被引量：4

Effect of Smac/DIABLO on the apoptosis and chemotherapy sensitivity of SW1990 cells of pancreatic cancer

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摘要目的研究Smac/DIABLO与胰腺癌对TRAIL和吉西他滨化疗敏感性的关系。方法构建Flagpc3.1-Smac外源表达质粒,在SW1990细胞中过表达SMAC,采用实时定量荧光PCR方法检测Smac mRNA水平;应用Western免疫印迹法检测caspase-3、caspase-9和Bcl-2蛋白水平;利用MTT法检测转染Flag-pc3.1-Smac和TRAIL、吉西他滨处理后SW1990细胞增殖能力的改变;利用流式细胞术检测转染Flag-pc3.1-Smac后SW1990细胞的凋亡情况。结果转染Flag-pc3.1-Smac后,SW1990细胞中Smac mRNA和蛋白质表达水平显著高于对照组;MTT试验显示,过表达Flag-pc3.1-Smac的SW1990细胞在1~5 d的培养期中490 nm处吸光值均低于对照组。结论 Smac/DIABLO促进胰腺癌SW1990细胞凋亡,并增加对TRAIL和吉西他滨的化疗敏感性。 Objective To observe the effect of Smac/DIABLO on the chemotherapy sensitivity of pancreas cancer to TRAIL and gemcitabine.Methods Real-time PCR was used to analyze the expression of Smac in SW1990 cells transfected with control and Flag-pc3.1-Smac plasmids respectively.Western blotting was used to detect the levels of caspase-3,caspase-9 and Bc1-2 protein.MTT was used to evaluate the growth of cells.Flow cytometry was used to detect the apoptosis of SW1990 cells transfected with control and Flag-pc3.1-Smac plasmids respectively.Results The mRNA and protein expression of Smac/DIABLO was higher in SW1990 cells transfected with Flag-pc3.1-Smac than that of control cells.SW1990 cells transfected with Flag-pc3.1-Smac had lower absorption at 490 nm than control SW1990 cells.Conclusion Smac/DIABLO promotes the apoptosis of pancreas cancer SW1990 cell and enhances the chemotherapy sensitivity to TRAIL and gemcitabine.

作者宏峰曾淑媛钟碧青

机构地区广东省博罗县人民医院药剂科

出处《实用药物与临床》 CAS 2016年第2期140-143,共4页 Practical Pharmacy and Clinical Remedies

关键词 SMAC/DIABLO TRAIL 吉西他滨胰腺癌化疗敏感性 Smac/DIABLO TRAIL Gemcitabine Pancreas cancer Chemotherapy sensitivity

分类号 R735.9 [医药卫生—肿瘤]

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