分蘖洋葱-番茄套作系统中番茄叶片差异蛋白表达分析

Differential Protein Analysis of Tomato Leaves in Tomato-Potato Onion Intercropping System

【目的】明确套作对番茄叶片差异蛋白表达的影响,从蛋白质水平进一步揭示套作分蘖洋葱减轻番茄连作障碍的机理,探明套作控病增产的原因,推动番茄高效优质栽培模式的建立和推广。【方法】以番茄为主栽作物,分蘖洋葱为套作作物,番茄叶片为试材,将4个不同化感潜力的分蘖洋葱品种(化感潜力强的‘绥化’和‘五常红七社’,化感潜力弱的‘宁安市红城村’和‘七台河’)与番茄套作,运用蛋白质双向电泳和MALDI-TOF-MS质谱鉴定技术,鉴定套作不同化感潜力分蘖洋葱对番茄叶片蛋白质表达谱的变化,分析差异蛋白的种类和功能。【结果】套作后不同处理番茄叶片共鉴定出39个差异表达蛋白,分为7类,包括光合作用相关蛋白、代谢相关蛋白、能量代谢相关蛋白、植物抗性相关蛋白、蛋白质合成相关蛋白、核酸合成相关蛋白和未知功能蛋白,差异较大的是与光合(11个)、代谢(11个)、植物抗性(8个)相关的蛋白。套作处理后,热激蛋白、硫氧还蛋白、多酚氧化酶、景天庚酮糖-1,7-二磷酸酶、S-腺苷硫氨酸脱氢酶、ATP结合蛋白在番茄叶片中的表达量均有不同程度上调,化感潜力强的品种表达量显著高于弱的品种。【结论】套作分蘖洋葱对番茄叶片的蛋白表达存在差异,且化感潜力强的品种差异蛋白表达量高于化感潜力弱的品种,表明套作化感潜力强的品种有利于促进番茄的生长,增强其抗逆性,从而减轻连作障碍。

【Objective】The objective of this study was to better understand the soil sickness mechanism of the tomato intercropped with four different allelopathic potential potato onions which has high yield and less plant diseases, obtain the differential expressed proteins of tomato leaves in tomato-potato onion intercropping system, and improve the foundation and extend of tomato cultivation mode.【Method】 A comparative proteome analysis was conducted to study the impact of tomatoes under normal monoculture and intercropping with four varieties of potato onion with different allelopathic potential（high allelopathic potential cultivars： ‘Suihua＇, ‘Wuchanghongqishe＇, and low allelopathic potential cultivars： ‘Ninganhongcheng＇, ‘Qitaihe＇）. Total proteins were extracted from these tomato leaves, and subjected to two-dimensional electrophoresis, followed by MALDI-TOF-MS mass spectrometry. Types and functions of proteins differently expressed in the leaves were analyzed.【Result】The results showed that 39 proteins differently expressed in the leaves of intercropped tomato. These proteins were divided into main 7 categories, including proteins related to photosynthesis, metabolism, energy metabolism, plant resistance, protein synthesis, nucleic acid synthesis and unknown protein. The proteins showing a higher abundance were primarily involved in 11 photosynthesis proteins, 10 metabolic proteins and 8 plant resistance proteins discovered. The photosynthesis of intercropped tomato enhanced and resistancealso increased. The expression levels of heat shock-related protein, thioredoxin peroxidase, polyphenoloxidase, sedoheptulose-1,7-bisphosphatase, S-adenosylmethionine synthase, ATP-binding protein increased in the leaves of intercropped tomato. Interestingly, the expression levels of these proteins from the tomato intercropped with potato onions with high allelopathic potentials were significantly higher than that intercropped with potato onions with low allelopathic potentials.【Conclusion】Our results showed that potato onion with different allelopathic potentials intercropped have different influences on tomato at the protein expression. The tomato intercropped with potato onions with high allelopathic potentials were stronger than tomato intercropped with potato onions with low allelopathic potentials, and is an effective way for promoting tomatoes healthy growth and better plant resistance.