不同营养时期BCMO1基因mRNA在牦牛组织中特异性表达的研究

Study on the tissue-specific mRNA expression of the BCMO1 gene in yaks during different nutritional periods

为了研究营养丰盛期和匮乏期β-胡萝卜素-15,15'-单加氧酶(BCMO1)基因mRNA表达水平,探讨BCMO1基因mRNA的组织特异性表达规律,试验采用实时荧光定量PCR检测营养匮乏期和营养丰盛期牦牛各组织中BCMO1基因mRNA表达量。结果表明:在营养匮乏期,BCMO1基因mRNA在肺脏、肝脏、肾脏、空肠、十二指肠、肌肉、胰腺、瘤胃8个组织中均有表达,且在十二指肠中的表达量最高(P<0.05),其次是肾脏、空肠、肌肉和瘤胃,并高于胰腺、回肠、肝脏和肺脏(P<0.05);在营养丰盛期,BCMO1基因mRNA在十二指肠中的表达量最高(P<0.05),其次是胰腺、肾脏和肌肉,胰腺中mRNA的表达量高于肾脏(P<0.05),而肾脏高于肌肉(P<0.05);空肠、回肠和瘤胃的表达量差异不显著(P>0.05)。从转录水平看,牦牛不同时期各组织BCMO1基因mRNA表达存在差异,营养匮乏期BCMO1基因mRNA的表达高于营养丰盛期,表明β-C的代谢产物(视黄酸等)可能会反馈性抑制BCMO1基因mRNA的表达。

To study the mRNA expression levels of β- carotene- 15,15'- monooxygenase( BCMO1) gene during the nutrient- rich and nutrient- deficiency periods,and investigate the tissue- specific mRNA expression pattern of the BCMO1 gene,a real- time fluorescent quantitative PCR was used to detect mRNA expression levels of the BCMO1 gene in yak tissues during the nutrient- deficiency and nutrient- rich periods. The results showed that the mRNA of the BCMO1 gene was expressed in eight tissues during the nutrient- deficiency period,including the lung,liver,kidney,jejunum,duodenum,muscle,pancreas,and rumen. The mRNA expression level in the duodenum was highest( P <0. 05),followed by in the kidney,jejunum and muscle,and rumen,which were significantly higher( P < 0. 05) than those in the pancreas,ileum,liver,and lung. The mRNA expression level of the BCMO1 gene in the duodenum was highest( P < 0. 05) during the nutrient- rich period,followed by in the pancreas,kidney and muscle,and the mRNA expression level in the pancreas was significantly higher( P < 0. 05) than that in the kidney,which was significantly higher( P < 0. 05) than that in the muscle. There were no significant differences( P > 0. 05) in the mRNA expression levels the BCMO1 gene in jejunum,ileum and rumen. There were differences in the mRNA expression levels of the BCMO1 gene among different tissues of yaks during different nutritional periods,and the mRNA expression levels of the BCMO1 gene during nutrient-deficiency period were higher than those during the nutrient- rich period based on the transcript level. The results indicate that β- C metabolites( such as retinoic acid,etc.) may have a feedback inhibition on the mRNA expression of the BCMO1 gene.