薄层扫描法测定补阳还五胶囊黄芪甲苷的含量

Determination of Astragaloside Ⅳ in BuYang HuanWu Capsules by TLC

目的：建立薄层扫描法测定补阳还五胶囊黄芪甲苷含量的方法。方法：硅胶G薄层板,氯仿-甲醇-水（13∶6∶2）10℃以下放置过夜的下层溶液展开,λ_S=530 nm,λ_R=700 nm。结果：线性范围为0.980~6.860μg,平均回收率为99.49%,精密度RSD为0.846%。结论：该方法简便,灵敏度高,重复性好,回收率高,可用于补阳还五胶囊中黄芪甲苷的含量测定。

Objective： To establish the determination method of astragaloside Ⅳ in BuYang HuanWu capsules by thin layer scanning（TLC）. Methods： Silica gel G TLC-scanner, loroform-methanol-water（13：6：2）, placed below10℃ and stayed overnight, the lower solution was taken out and spread, λS=530 nm, λR=700 nm. Results： Linearity range was between 0.980 and 6.860 μg, average recovery rate was 99.49%, precision RSD was 0.846%. Conclusion：The method, simple, sensible, reproducible and with high recovery rate, could be used to content determination of astragaloside Ⅳ in BuYang HuanWu capsules.