摘要
目的探讨生长抑素Ⅳ型受体激动剂(NNC 26-9100)对异氟醚麻醉所致老年鼠学习记忆障碍的改善作用。方法将20月龄老年SD大鼠随机分为4组(n=14):对照组、异氟醚组、异氟醚+药物组和单纯药物组。异氟醚+药物组和单纯药物组侧脑室注射NNC 26-9100,而对照组和异氟醚组注射等量溶媒。24 h后异氟醚组和异氟醚+药物组吸入2%异氟醚4 h,而对照组和单纯药物组吸入纯氧4 h。通过Morris水迷宫检测记忆能力、real-time PCR和Western blot检测中性内肽酶(NEP)和胰岛素降解酶(IDE)在海马区表达。结果与异氟醚组相比,异氟醚+药物组大鼠第2天及第3天逃避潜伏期缩短(P<0.05),目的象限探索时间延长(P<0.05)。各组海马区NEP和IDE在mRNA及蛋白水平两两比较均无显著差异。结论生长抑素Ⅳ型受体激动剂(NNC-9100)可显著改善异氟醚麻醉所致老年鼠学习记忆障碍。
Objective To investigate the effect of somatostatin receptor subtype-4 agonist （NNC 26-9100 ） on learning and memory impairment in aged rats induced by isoflurane anesthesia. Methods Sprague Dawley rats were randomly divided into 4 groups （ n = 14） as follows ： V ＋ O2, V ＋ ISO, NNC ＋ ISO and NNC ＋ 02. The NNC ＋ ISO and NNC ＋ Oz groups received bilateral intracerebroventricular injection of NNC 26-9100. The V ＋ 02 and V ＋ ISO groups received equal volume of vehicle control. 24 hours after injection, V ＋ ISO group and NNC ＋ ISO group inhaled 2% isoflurane for 4 hours, while the rest group received 4 hr of 100% 02. Memory was assessed in the Morris water maze. The expression of neprilysin（NEP） and insulin degrading enzyme（IDE） in hippocampus were measured by real-time PCR and Western blot. Results Compared with V ＋ ISO group, rats in NNC ＋ ISO group took less time to find platform in day 2 and 3 （ P 〈 0.05 ）, spent more time in the target quadrant and crossed more number of platform（ P 〈0. 05 ）. There was no significant difference in expression level of NEP and IDE in each group. Conclusions Somatostatin receptor subtype-4 agonist （ NNC 26-9100） can improveisoflurane-induced learning and memory dysfunction.
出处
《基础医学与临床》
CSCD
2016年第3期337-341,共5页
Basic and Clinical Medicine
基金
国家自然科学基金(81371205)
