乙肝肝硬化患者血清CTHRC1及TGF-β_1相关性研究

Correlation between serum level of CTHRC1 and TGF-β_1 in patients with hepatitis B cirrhosis

目的研究乙肝病毒(HBV)致肝硬化患者血清胶原三股螺旋重复蛋白1(CTHRC1)表达水平,分析其与疾病病程的关系。方法HBV感染确诊肝硬化患者,测定总胆红素(TBIL),清蛋白(ALB),凝血酶原时间(PT),根据测定结果对患者进行评分,分级为A、B、C三级,分为三组,测定各组透明脂酸酶(HA),层粘连蛋白(LN),Ⅲ型前胶原氨基端肽(PⅢNP),Ⅳ型胶原(CⅣ)血清浓度以了解各组患者肝纤维化状态,B超检测三组患者门静脉内径以了解各组肝脏功能状态,采用ELISA法检测三组患者血清CTHRC1,转化生长因子β1(TGF-β1)浓度。结果 B、C组HA、LN、PⅢNP、CⅣ均明显高于A组和对照组,差异有统计学意义(P<0.05),A组与对照组各指标浓度差别不大,差异无统计学意义(P>0.05);各组肝硬化患者门静脉内径均明显大于对照组,B组与A组,C组与A组比较,差异均有统计学意义(P<0.05);A、B、C组CTHRC1及TGF-β1浓度均高于对照组,且随着临床分期分值升高而升高,差异有统计学意义(P<0.05)。结论随着肝硬化患者临床分期进展及肝功能下降,血清CTHRC1及TGF-β1浓度上升,两者具有一定的相关性,其机制需要进一步研究。

Objective To research the serum expression level of collagen triple helix repeat containing 1（CTHRC1）in the patients with HBV caused cirrhosis,and to analyze its relationship with disease course.MethodsTBIL,ALB and PT were detected in the patients with definitely diagnosed cirrhosis caused by HBV infection.Then the patients were scored according to the detecting results and divided into the grade A,B and C.The serum levels of HA,LN,PⅢNP and CⅣ were detected in 3groups for understanding the liver fibrosis status.B-ultrasound was performed to detect the portal vein diameter for understanding the liver function state in each group.The serum CTHRC1 and TGF-β1levels were detected by ELISA.Results The levels of HA,LN,PⅢNP and CⅣin the group B and C were significantly higher than that in the group A and the control group,the differences were statistically significant（P〈0.05）,but which had little differences between the group A and the control group,showing no statistical significance（P〈0.05）;the portal vein diameter of the liver cirrhosis patients in each group was significantly greater than that in the control group,which had statistical difference between the group B and A and between the group C and A（P〉0.05）;the CTHRC1 and TGF-β1levels in the group A,B and C were higher than those in the control group,moreover which were increased with the clinical stage elevation,the difference was statistically significant（P〈0.05）.Conclusion Along with the clinical staging progress and liver function decline in the patients with liver cirrhosis,serum CTHRC1 and TGF-β1levels are increased,both has certain correlation,but its mechanism needs further research.