甲巯咪唑对成人Graves病患者外周血淋巴细胞活化状态的影响

Effects of methimazole treatment on human peripheral blood lymphocytes activation in adolescents with Graves' disease

目的探讨甲巯咪唑对Graves病(GD)甲状腺功能亢进治疗前后外周血淋巴细胞活化率的影响。方法通过流式细胞仪检测50例GD初诊患者甲巯咪唑(MMI)治疗前后及20例健康对照者淋巴细胞活化标志物(CD25,CD69)表达水平,初步分析MMI在GD治疗中的免疫调控机制。结果GD初诊组患者淋巴细胞活化率显著高于健康对照组及MMI治疗组(P<0.01),而MMI治疗组与健康对照组相比,除CD8+CD25+细胞外其余指标均无显著差异(P=0.04)。结论活化的淋巴细胞在GD发生发展过程中扮演着重要角色,MMI可能通过抑制淋巴细胞活化而改善GD患者免疫状态。

Objective To assess the frequencies of activated CD4＋ and CD8＋ T lymphocytes and B lymphocytes in adolescents with hyperthyroidism due to Graves＇ disease（GD）, and to assess changes in the above-mentioned parameters during methimazole treatment. Method CD25 and CD69 expression levels were detected by flow cytometry in 50 GD patients before and after treatment with methimazole（MMI） and 20 cases of healthy controls, preliminary analyzed the immune regulation mechanism of MMI in the treatment of GD. Result The activation rate of lymphocytes in GD patients with initial diagnosis group was significantly higher than healthy controls group and GD patients treated with MMI（P〈0.01）. After treatment, no vital differences in percentages of activated cells between GD patients and controls were found, except CD8＋CD25＋cells（P = 0.04）. Conclusion The present study demonstrates that both activated T and B cells might play an important role in the pathogenesis of GD. The use of MMI in the treatment of hyperthyroidism due to GD leads to decrease the frequencies of activated lymphocytes.