二十二碳六烯酸对慢性氯胺酮暴露所致空间学习记忆损害的干预效果的行为学研究

THE BEHAVIORAL INFLUENCE OF DHA ON THE DAMAGES OF SPATIAL LEARNING AND MEMORY OF CHRONIC KETAMINE EXPOSURE MICE

目的:本研究主要观测了二十二碳六烯酸对慢性氯胺酮暴露所致ICR小鼠的空间学习记忆功能障碍的行为学影响。方法:使用Morris水迷宫评估小鼠的空间学习记忆能力,小鼠腹腔注射氯胺酮(30 mg/kg/次,每日2次)28 d造成慢性氯胺酮暴露导致空间认知损害的模型,此后灌胃二十二碳六烯酸[420 mg/(kg·d)]42 d后观察实验小鼠空间认知功能的改善情况。结果:(1)氯胺酮处理后的小鼠定向航行试验和空间探索试验的表现明显差于对照组。(2)氯胺酮组小鼠定向航行试验和空间探索实验中的表现差于氯胺酮+DHA组。结论:慢性氯胺酮暴露导致了小鼠空间认知功能损害,二十二碳六烯酸可以在一定程度上改善ICR小鼠慢性氯胺酮暴露所致的空间学习损害。

Objective： The aims of this study are to evaluate the behavioral influence of docosahexenoic acid on learing and memory dysfunction induced by chronic ketamine exposure in ICR mice.Methods： In our study,the spatial learning and memory ability of ICR mice were evaluated by Morris water maze.First,ketamine was administered intraperitoneally （twice per day,each dose was 30mg·kg-1） for 28 days to establish animal model of spatial learning and memory damages.Then,mice were fed with docosahexaenoic acid（ 420 mg / kg / d） for 42 days, and the change of behavioral performance in experimental mice were observed.Results：（1） The performance of ketamine treated mice were significantly worse than control mice in training phase and probe test of Morris water maze test.（2）For ketamine-treated mice,the behavioral performance of mice followed by DHA administered were significantly better than those which followed by saline administrered.Conclusion： Chronic ketamine exposure leads to spatial learning and memory dysfunction in ICR mice,however,docosahexaenoic acid possibly can improve this spatial learning and memory dysfunction to some extent.