根管冲洗液对镍钛锉表面形貌影响研究

Effect of root canal irrigation on the surface morphology of nickel-titanium instruments

目的研究两种根管冲洗液对镍钛锉表面微观形貌的影响。方法于2015年7—8月在南京大学化学院进行研究。选取未经冲洗液浸泡的MTwo新锉2根,使用原子力显微镜(AFM)扫描观察,然后将2根锉分别在5.25%NaOCl和17%EDTA溶液中动态浸泡10 min,之后再次使用AFM扫描。通过AFM自带软件分析器械表面粗糙度并生成三维图像,记录并比较冲洗液浸泡前后的MTwo锉平均粗糙度值(Ra)和均方根值(RMS)。结果5.25%NaOCl溶液浸泡后的MTwo锉,其Ra和RMS值均高于浸泡前的新锉,浸泡前、后差异具有统计学意义(P<0.05);17%EDTA溶液浸泡前后的MTwo锉Ra和RMS值比较,则差异无统计学意义(P>0.05)。AFM三维图像显示,无论是新锉还是冲洗液浸泡过的锉,其纳米结构上的表面形貌均不规则。结论 AFM是量化分析冲洗液对根管器械表面微观形貌影响较佳的一种方式。5.25%NaOCl溶液浸泡会增加MTwo锉的表面粗糙度,而17%EDTA溶液浸泡未使MTwo锉表面结构产生改变。

Objective To study the effect of root canal irrigation on the surface morphology of nickel-titanium instruments.Methods The study was proceeded in Chemistry College of Nanjing University from July 2015 to August 2015.Two new MTwo files without any immersion were subjected to atomic force microscopy（AFM）analysis;then the instruments were dynamically immersed in 5.25% NaOCl and 17% EDTA solutions for 10 min,respectively,thereafter subjected to AFM analysis again. By the software of AFM,the surface roughness was analyzed and three-dimensional images were generated. Record and compare the Ra and RMS values of MTwo file before and after immersion.Results After5.25%NaOCl immersion,there was a statistically significant increase in Ra and RMS values. The difference was not statistically significant in Ra and RMS values after 17% EDTA immersion. Three-dimensional images of AFM displayed that the surface morphology of nanostructures was irregular both in the new file and the file after immersion.Conclusion AFM is preferred as a way to quantitatively analyze the influence of irrigation on the surface morphology of endodontic instruments. The 5.25% NaOCl solution immersion significantly increases the surface roughness of the MTwo instruments,whilst 17% EDTA solution does not alter the surface structure of MTwo file.