骨皮质切开术辅助大鼠正畸牙移动中M-CSFmRNA,ANKLmRNA,OPGmRNA的研究

Expression of M-CSFmRNA, RANKLmRNA and OPGmRNA in rats during corticotomy-assisted orthodontic tooth movement

目的:观察M-CSF(巨噬细胞集落刺激因子),RANKL(核激活因子KB受体活化因子配体),Osteoprotegerin(骨保护蛋白)在大鼠骨皮质切开术辅助正畸牙移动过程中的表达情况。方法:选取健康雄性Wistar大鼠65只,60只大鼠随机分成3组:组Ⅰ:选择性骨皮质切开术组;组Ⅱ:传统牙齿移动组;组Ⅲ:混合组,剩余5只作为空白对照组(不作任何处理)。用镍钛螺旋拉簧施加约60g力近中移动上颌实验侧第一磨牙,在第一磨牙颊腭侧行打孔式骨皮质切开术,分别在加载第7、14、21、28、42d颈椎脱臼处死取材,应用SYBR Green实时荧光定量技术和MxPro-Mx3000P软件对取材进行分析。结果:组Ⅲ的破骨细胞主要调节因子和生化标记物:M-CSF(巨噬细胞集落刺激因子),RANKL(核激活因子KB受体活化因子配体)的表达与组Ⅰ和组Ⅱ相比增加,暗示组Ⅲ骨吸收活跃。组Ⅲ与组Ⅱ相比,呈现出独特的牙槽骨改建模式:藕联的RANKL和OPG促进牙槽骨改建。结论:正畸牙齿移动初期骨皮质切开术加速了牙齿移动,改变了早期骨代谢状态,破骨活跃,支持区域性骨代谢加速学说。

Objective： To study the expression of M-CSF, RANKL and OPG in rats during corticotomy assisted orthodontic tooth movement. Methods： 65 eight-week healthy male Wistar rats were recruited： 60 were randomly divided into3 groups： Group I for selective corticotomy, Group II for traditional tooth movement, Group III for mixed group; the five remaining as control group. 6og tensile strength was placed mesially on the first molar with a Ni-Ti spring. Drilling operation was performed on the buccal and palatal bone surfaces of the left maxillary first molar tooth during the procedure of surgery corticotomy. On days 7, 14, 21, 28 and 42, after the rats were sacrificed by cervical dislocation, the specimen were prepared and analyzed by using the SYBR Green real-time fluorescent quantitative PCR technology to observe the changes of biochemical indicators for bone reconstruction during tooth movement after surgical corticotomy. Results： RNA markers of osteoclastic cells and key osteoclastic regulators（M-CSF[macrophage colony-stimulating factor], RANKL [receptor activator of nuclear factor kappa-B ligand]） all showed expression indicating increased osteoclastogenesis in the combined side, the combination approach represents a unique pattern of challenge to the alveolar bone in which the coupling of RANKL and OPG is more rapid and simultaneous compared with conventional tooth movement, where an increase in RANKL was associated with decreased OPG. Conclusion： The corticotomy enhances the rate of tooth movement during the initial tooth displacement phase, changes the early bone metabolic state and enhances bone resorption. The study supports the theory of regional Acceleratory Phenomenon.