鸦胆子油乳对膀胱癌影响的实验分析

目的通过实验分析鸦胆子油乳对膀胱癌的影响。方法在人体外培养人膀胱癌细胞(BIU-87),并将不同浓度的鸦胆子油乳加入其中,之后观察人膀胱癌细胞的生长、组织结构和细胞周期。对ICR小鼠进行亚硝胺的膀胱癌诱导,再给ICR小鼠膀胱灌注鸦胆子油乳,在光镜和电镜下观察膀胱癌的进展或抑制情况。结果鸦胆子油乳对人膀胱癌细胞产生了抑制作用,并破坏了膀胱癌细胞的微结构和超微结构,改变了膀胱癌细胞的性质并致其坏死,同时它还能够阻止人膀胱癌细胞由G0期向S期进展以及抑制DNA的合成。ICR小鼠实验结果表明,亚硝胺诱导的膀胱癌在膀胱灌注鸦胆子油乳之后得到有效的抑制。结论鸦胆子油乳能够抑制膀胱癌细胞的生长与繁殖,临床中可加以应用。

objective Through experimental analysis of Brucea javanica oil emulsion on bladder cancer. Methods Cultured human bladder cancer cells outsidethe body （BIU-87）, and different concentration of Brucea javanica oil emulsion added, after the observation of human bladder cancer cell growth, tissue structureand cell cycle. Of nitrosamines in ICR mice urinary bladder cancer induced by ICR in mice, then to intravesical instillation of Brucea javanica oil emulsion,under light microscope and electron microscope observation of bladder cancer or inhibition. Results Brucea javanica oil emulsion produced an inhibitory effecton human bladder cancer cell, and damage to the bladder cancer cell membrane, mitochondrial membrane, endoplasmic reticulum, nuclear membrane system,changed the nature of bladder cancer cells and its induced necrosis, it also can prevent the synthesis of human bladder cancer cells from G0 phase to S phaseprogression and the inhibition of DNA. The experimental results show that ICR mice, nitrosamine induced bladder cancer can be effectively suppressed afterintravesical instillation of Brucea javanica oil emulsion. Conclusion The growth and reproduction of Brucea javanica oil emulsion can inhibit bladder cancer cellscan be used in clinical.