miR-135b、LZTS1、β-catenin在胰腺癌中的表达及临床意义

Clinical significance of expression of miR-135b,LZTS1 and β-catenin in pancreatic cancer

目的:探讨miR-135b、LZTS1及β-catenin在胰腺癌中的表达意义及三者之间的相关性.方法:分别采用锁定核酸原位杂交技术(LNA-ISH)和免疫组织化学法检测miR-135b、LZTS1和β-catenin蛋白在70例胰腺癌及相应癌旁组织中的表达情况.结果:miR-135b在胰腺癌中的表达率高于癌旁正常组织(71.4%vs 42.9%,P=0.001),LZTS1、β-catenin蛋白在胰腺癌中的表达率均低于癌旁正常组织(34.3%vs 68.6%、34.3%vs 74.3%,P<0.05).在胰腺癌组织中miR-135b与LZTS1的表达水平呈负相关(r=-0.61,P<0.05),与β-catenin无相关性(r=0.06,P>0.05);LZTS1与β-catenin呈正相关(r=0.37,P<0.05),且miR-135b、LZTS1和β-catenin的阳性表达水平与胰腺癌的临床分期及淋巴结转移密切相关(P<0.05),与胰腺癌患者年龄、性别、肿瘤部位及组织学分级无关(P>0.05).结论:miR-135b在胰腺癌中表达升高,LZTS1与β-catenin在胰腺癌中表达减少,并且表达升高的miR135b可能通过下调LZTS1基因表达而参与了胰腺癌的发生发展.

AIM： To detect the expression of miR-135b, LZTS1 and β-catenin in pancreatic cancer in an attempt to explore their clinical significance. METHODS： Locked nucleic acid in situ hybridization （ISH） and immunohisto- chemistry were used to detect the expression of miR-135b, LZTS1 and β-catenin proteins in 70 pancreatic cancer tissues and adjacent normal tissues, respectively. RESULTS： The positive rate of miR-135b in pancreatic cancer was higher than that in adjacent tissues （71.4% vs 42.9%, P = 0.001）. The positive rates of LZTS1 and β-catenin proteins in pancreatic cancer were significantly lower than those in adjacent tissues （34.3% vs 68.6%, 34.3% vs 74.3%, P 〈 0.05）. Expression of miR-135b had a negative correlation with that of LZTS1 in pancreatic cancer （r = -0.61, P 〈 0.05）, but miR-135b expression had no significant correlation with 13-catenin （r = 0.06, P 〉 0.05）. LZTS1 expression had a positive correlation with that of β-catenin （r = 0.37, P 〈 0.05）. Expression of miR-135b, LZTS1 and 13-catenin was closely related with lymph node metastasis and clinical stage （P 〈 0.05）, but had no correlation with patient age, sex, tumor site or histological grade （P 〉 0.05）.CONCLUSION： miR-135b is highly expressed in pancreatic cancer, while the expression of LZTS1 and β-catenin is decreased. Up- regulated expression of miR-135b may participate in the development of pancreaticcancer by down-regulating the expression of LZTS1 protein.