采用ERIC-PCR与PFGE分析鲍曼不动杆菌基因型和同源性并对比方法学差异

Comparison between PFGE and ERIC-PCR for genotyping and homologous analysis of Acinetobacter baumannii

目的比较脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)与肠杆菌科基因间重复序列聚合酶链式反应(enterobacterial repetitive intergenic consensus-polymerase chain reaction,ERICPCR)检测鲍曼不动杆菌同源性的结果差异。方法分别采用PFGE和ERIC-PCR对我院院内分离的43株鲍曼不动杆菌进行分型检测。结果 43株鲍曼不动杆菌通过PFGE分型得出:A型22株、B型10株、C型3株、D型4株、E型2株、F型1株、G型1株;通过ERIC-PCR得出7种型别:Ⅰ型22株、Ⅱ型10株、Ⅲ型3株、Ⅳ型2株、V型3株、Ⅵ型1株、Ⅶ型2株。2种方法结果相符率为76.8%。我院鲍曼不动杆菌存在克隆株传播。结论 ERIC-PCR操作简便、结果可靠,与PFGE结果一致性高,2种分型方法均适合作为医院进行病原菌流行病学调查的分型检测手段。

Objective To compare the pulsed field gel electrophoresis（PFGE） with the enterobacterial repetitive intergenic consensus polymerase chain reaction（ERIC-PCR） in genotyping and homologous analysis of Acinetobacter baumannii. Methods PFGE and ERIC-PCR were used to test the genotypes of 43 isolates of Acinetobacter baumannii, respectively. Results 43 isolates of Acinetobacter baumannii were classified into 7 genotypes by PFGE, including genotype A in 22 samples, genotype B in 10,genotype C in 3, genotype D in 4, genotype E in 2, genotype F in 1 and genotype G in 1. Correspondingly,they were categorized by using ERIC-PCR method as following 7 genotypes： typeⅠin 22, typeⅡ in 10, typeⅢ in 3, type Ⅳ in 2, type Ⅴ in 3, typeⅥ in 1 and type Ⅶ in the remaining 2 samples. The correlation coefficient between the two methods was 76.8%. It was suggested that there were spreads of clonal Acinetobacter baumannii among the patients in our hospital. Conclusion ERIC-PCR is a simple and reliable method of bacterial genotyping. The data are highly correlated with the results acquired by PFGE. Both methods are suitable for applying in the epidemiological investigation of nosocomial infections.