类风湿性关节炎患者外周血T细胞RANKL增加且血清Dickkopf1降低

RANKL increases on peripheral blood T lymphocytes and serum Dickkopf1 decreases in rheumatoid arthritis

目的探讨骨代谢相关分子在类风湿性关节炎(RA)患者骨代谢异常和疾病进展过程中的作用。方法纳入RA患者66例及健康对照20例,搜集相关临床信息。利用电化学发光免疫分析法检测受试者血清中骨钙素N端中段(OC-N-MID)和1型胶原蛋白交联羧基末端肽(CTX)水平。采用液相悬浮芯片法检测Wnt抑制因子Dickkopf1(DKK1)、核因子κB受体激活蛋白配体(RANKL)含量。利用流式细胞术检测外周血T细胞上RANKL的水平。结果与健康对照组相比,RA患者血清中OC-N-MID、CTX含量无显著性差异,RANKL水平升高,DKK1水平降低。RA患者外周血T细胞上RANKL水平增加,尤其CD3+T细胞亚群上RANKL增加显著。结论 RA患者T细胞上RANKL水平增加,血清中RANKL含量升高,DKK1水平降低。

Objective To explore the roles of bone metabolism-related molecules in bone metabolic disturbance and disease progression of patients with rheumatoid arthritis（ RA）. Methods A total of 66 RA patients and 20 healthy controls were included,and their relevant clinical information was gathered. Electrochemiluminescence immunoassay（ ECLIA） was used to detect the levels of osteocalcin N-terminal middle（ OC-N-MID） and C-terminal cross-linked telopeptides of type 1collagen（ CTX） in serum. The serum levels of Wnt inhibitory factor Dickkopf1（ DKK1） and receptor activator of nuclear factor-κB ligand（ RANKL） were determined by magnetic luminex assays. Flow cytometry was used to detect the RANKL level on peripheral blood T cells. Results Compared with healthy controls,the levels of OC-N-MID and CTX in the sera of the RA patients showed no significant difference. Level of RANKL in the sera of the RA patients was higher than that in the controls,while level of DKK1 was lower. The level of RANKL on peripheral blood T cells increased in the RA patients,especially on CD3＋T cells. Conclusion In RA patients,level of RANKL on T cells is elevated,and the serum level of RANKL increases,but DKK1 decreases.