自发性高血压大鼠外周血CD4^+/CD8^+T淋巴细胞比率增加且连接子蛋白40(Cx40)和炎症因子水平升高

An increase in the ratio of peripheral blood CD4^+/CD8^+T lymphocytes and the levels of connexin 40 and inflammatory factors in spontaneously hypertensive rats

目的探讨连接子蛋白40(Cx40)与自发性高血压大鼠(SHR)外周血CD4^+T细胞和CD8^+T淋巴细胞亚群及炎症因子之间的关系。方法采用流式细胞术检测Wistar京都(WKy)大鼠和SHR外周血CD4^+T细胞和CD8^+T细胞及其表面Cx40的表达;采用ELISA检测炎症因子白细胞介素2(IL-2)、γ干扰素(IFN-γ)、IL-4、IL-6表达。结果 SHR收缩压显著高于WKy大鼠,SHR外周血CD4^+T细胞百分率、CD4^+/CD8^+T细胞比率显著高于WKy大鼠,而CD8^+T细胞百分率显著低于WKy大鼠;SHR血清IL-2、IL-4、IL-6水平均显著高于WKy大鼠,IFN-γ无明显差异;SHR外周血CD4^+T细胞和CD8^+T细胞中Cx40表达均显著高于WKy大鼠。结论 SHR外周血CD4^+/CD8^+T淋巴细胞比率增加,且Cx40和IL-2、IL-4、IL-6水平明显升高。

Objective To analyze the relationships of connexin 40（ Cx40） with peripheral blood CD4^＋ and CD8^＋ T lymphocyte subsets and inflammatory factors of spontaneously hypertensive（ SH） rats. Methods Flow cytometry was used to detect CD4^＋,CD8^＋lymphocytes and Cx40 expression on the cells in the peripheral blood of Wistar-Kyoto（ WKy） rats and SH rats. ELISA was performed to test the levels of interleukin 2（ IL-2）,interferon γ（ IFN-γ）,IL-4 and IL-6. Results Compared with WKy rats,the systolic blood pressure,the percentage of CD4^＋T lymphocytes,the expression of Cx40 on the surface of CD4^＋and CD8^＋T lymphocytes and the ratio of CD4^＋/CD8^＋in the peripheral blood of SH rats were significantly higher,with the exception of the percentage of CD8^＋lymphocytes which was lower. Also,we found that the serum levels of IL-2,IL-4 and IL-6 in the SH rats were significantly higher than those of WKy rats. However,there was no significant difference in the IFN-γ level between SH and WKy rats. Conclusion The ratio of peripheral blood CD4^＋/CD8^＋T lymphocytes and the levels of Cx40,IL-2,IL-4 and IL-6 are significantly elevated in SH rats.