摘要
目的研究不同浓度神经肽Y(NPY)对人脂肪干细胞(h ADSC)增殖及分化的影响。方法分离h ADSC,用(10^(-6)~10^(-15))mol/L NPY诱导刺激h ADSC,MTT法检测其增殖;分别用完全培养基、NPY和胰岛素处理h ADSC,油红O染色观察h ADSC的分化;Western blot法检测各组过氧化物酶体增殖物激活受体γ(PPARγ)、CCAAT增强子结合蛋白α(C/EBPα)、诱导细胞死亡的d FF45样效应因子C(Cidec)和受体相互作用蛋白140(RIP140)的表达水平。结果 (10^(-11)~10^(-15))mol/L NPY促进h ADSC增殖,(10^(-6)~10^(-10))mol/L NPY抑制h ADSC增殖;(10^(-7)、10^(-9)、10^(-11))mol/L NPY均诱导h ADSC分化,并增加PPARγ、C/EBPα蛋白表达,同时促进白色脂肪组织特异性标志Cidec、RIP140蛋白的表达。结论较低浓度NPY促进h ADSC增殖,较高浓度NPY抑制h ADSC增殖并促进其分化。
Objective To investigate the impact of neuropeptide Y( NPY) at different concentrations on the proliferation and differentiation of human adipose-derived stem cells( h ADSCs). Methods The h ADSCs were separated and incubated with NPY at a range of concentrations 10^-6- 10^-15 mol/L. MTT assay was used to detect cell proliferation. Following the treatment of complete medium,NPY and insulin separately,cell differentiation was observed by Oil red O staining. In addition,Western blotting was performed to exam the levels of peroxisome proliferator-activated receptor-γ( PPARγ),CCAAT/enhancer binding protein alpha( C/EBPα),cel death-inducing d FF45-like effector c( Cidec) and receptor interacting protein 140( RIP140). Results MTT assay revealed that 10^-11- 10^-15 mol/L NPY stimulated the proliferation of h ADSCs,while high-dose NPY( 10^-6- 10^-10 mol/L) inhibited the cell proliferation. NPY( 10^-7,10^-9,10^-11 mol/L) induced the differentiation of h ADSCs. The expression levels of related adipocyte markers( PPARγ,C/EBPα),mature white adipose tissue specific markers( Cidec,RIP140) increased at the presence of NPY( 10^-7,10^-9,10^-11 mol/L). Conclusion Low-dose NPY could stimulate the proliferation of h ADSCs, and high-dose NPY could inhibit the proliferation and promote the differentiation of h ADSCs.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2016年第2期185-190,共6页
Chinese Journal of Cellular and Molecular Immunology
基金
湖南省科技厅项目(2013SK3060)