甲胎蛋白阴性肝细胞癌中埃兹蛋白(ezrin)和SIX1蛋白表达水平的临床病理意义

Clinicopathological significance of ezrin and SIX1 protein expression in alpha fetoprotein-negative hepatocellular carcinoma

目的探讨埃兹蛋白（ezrin）和SIX1蛋白过表达在甲胎蛋白（AFP）阴性肝细胞肝癌（HCC）中的临床病理学意义。方法应用免疫组织化学En Vision法检测ezrin和SIX1蛋白在50例AFP阴性的HCC组织及其相应的癌旁肝组织中的表达水平,χ^2检验和Spearman等级相关分析检验两者表达的相关性。结果在50例AFP阴性的HCC组织中,ezrin和SIX1蛋白的阳性率分别为68.0%（34/50）和60.0%（30/50）,明显高于癌旁肝组织的阳性率,分别为38.0%（19/50）和26.0%（13/50）。χ^2检验显示ezrin和SIX1蛋白表达与肿瘤的TNM分期以及脉管浸润相关。Spearman等级相关分析显示ezrin和SIX1在AFP阴性HCC组织中的表达呈显著正相关。结论 Ezrin和SIX1蛋白在AFP阴性HCC组织中均高表达,两者的表达与肿瘤的TNM分期以及脉管浸润相关,并且两者在肝癌中的表达具有相关性。

Objective To investigate the clinicopathological significance of ezrin and SIX1 overexpression in alpha fetoprotein（ AFP）-negative hepatocellular carcinoma（ HCC）. Methods The En Vision immunohistochemical method was used to detect the protein expression of ezrin and SIX1 in the tumor tissues and corresponding adjacent normal liver tissues from 50 AFP-negative HCC patients. The correlations between the overexpression of ezrin and SIX1 and the prognosis of the patients with HCC were analyzed by Chi-square test and Spearman analysis. Results The positive rates of ezrin and SIX1 protein expression in AFP-negative HCC tissues were 68. 0%（ 34/50） and 60. 0%（ 30/50）,respectively,and they were significantly higher than the expression rates [38. 0%（ 19/50） and 26. 0%（ 13/50） ] in adjacent normal liver tissues.Chi-square test showed that the expression of both ezrin and SIX1 proteins were related to TNM stage and lymphovascular infiltration,and Spearman analysis revealed a positive correlation between ezrin and SIX1 expression in AFP-negative HCC.Conclusion Both Ezrin and SIX1 proteins are highly expressed in AFP-negative HCC,and significantly related with the TNM stage and lymphovascular infiltration of patients with AFP-negative HCC. In addition,there is a positive correlation between ezrin and SIX1 expression in AFP-negative HCC.