3种选择性分离培养基检测单核细胞增生李斯特菌效果比较

Comparison on the detection effects of Listeria monocytogenes with three kinds of selective culture media

目的为更好地进行食品中单核细胞增生李斯特菌检测工作,使检测工作更加便利、准确和灵敏,保障食品安全,防范食源性疾病的发生,开展此项目的研究。方法参照食品安全国家标准食品微生物检验GB 4789.30—2010单核细胞增生李斯特菌检验和GB 4789.28—2013培养基和试剂的质量要求。结果本次研究选用的3种培养基的3个评价指标(生长率、生长指数、特异性)均符合GB 4789.28—2013中的要求,并且通过实际样品的加标试验进行验证比较,2种显色培养基的检测效果优于PALCAM培养基。结论在实际检测样品中,应根据不同的样品和目的,灵活的选择培养基和培养条件及检测方法,以提高检测的准确性和效率,减少漏检。

Objective This project is carried out to make the detection of Listeria monocytogenes in food more convenient,accurate and sensitive,to ensure food safety,and to prevent the occurrence of food- borne illness. Methods Reference to the national food safety standards of food microbiological GB 4789. 30—2010 Detection of Listeria monocytogenes and GB 4789. 28—2013 the Quality Requirements of Media and Reagents. Results The quality of three kinds of media in this study conformed to the requirements of GB 4789. 28—2013 in productivity ratios and growth factor and specificity,and it was proved that the effect of two kinds of chromogenic media was better than PALCAM by the results of standard test of samples. Conclusion In the clinical sample detection,should choose media,culture conditions and detection methods according to the the diverse samples and different purposes,so as to improve the detection accuracy and efficiency,and to reduce the residual.