脂肪干细胞移植抑制脑缺血大鼠梗死灶周围组织Nogo-A的表达

Adipose-derived stem cell transplantation inhibits the expression of Nogo-A in the perilesional cortex of rats with focal cerebral ischemia

目的探讨脂肪干细胞(ADSC)对脑缺血大鼠梗死灶周围组织轴索生长抑制因子A(Nogo-A)表达的影响。方法分离培养大鼠ADSC,线栓法制作大鼠大脑中动脉栓塞模型。大鼠分为假手术组、模型组和ADSC组。ADSC组于造模成功1 d后侧脑室注射ADSC悬液,模型组注射PBS。各组分别于术后4 d、7 d和14 d分批处死,采用反转录PCR检测梗死灶周围组织Nogo-A mRNA水平、免疫组织化学染色法和Western blot法检测梗死灶周围组织Nogo-A蛋白水平。结果与假手术组相比,模型组和ADSC组各个时间点梗死灶周围组织Nogo-A表达水平明显增加;ADSC组各个时间点梗死灶周围组织Nogo-A表达水平较模型组明显降低。结论 ADSC移植可有效抑制脑缺血后大鼠梗死灶周围组织Nogo-A的表达从而促进神经功能的恢复。

Objective To explore the effect of adipose-derived stem cells（ ADSCs） transplantation on the expression of neurite outgrowth inhibitor-A（ Nogo-A） after cerebral ischemia in middle cerebral artery occlusion（ MCAO） rats. Methods ADSCs were harvested and purified,and then MCAO models were established in rats by the suture method. Rats were divided into sham operation group,MCAO model group and ADSCs-injected group. One day after MCAO,ADSCs were injected into the lateral ventricle of the ADSC group rats and the same does of PBS was given to model group rats. At day 4,7 and 14 after MCAO,the rats were decapitated to detect the Nogo-A expression in the perilesional cortex by reverse transcription PCR,immunohistochemistry and Western blotting. Results Compared with sham group,the expression of Nogo-A was significantly up-regulated at each time point in both model group and ADSC group. Compared with model group,the expression of Nogo-A was significantly down-regulated at each time point in ADSC group. Conclusion ADSC transplantation could promote the recovery of neurological functions,partly by inhibiting the expression of Nogo-A in MCAO rat＇s brain.