调衡方多糖增强体外培养巨噬细胞的吞噬能力

Macrophage phagocytosis enhanced by Tiaohengfang polysaccharides in vitro

目的研究调衡方多糖对巨噬细胞吞噬功能的影响,探讨其多糖对巨噬细胞吞噬作用的免疫调节机制。方法依照中药多糖的制备方法从调衡方中提取粗多糖;从小鼠腹腔分离提取并培养巨噬细胞;(500、200、10)μg/m L调衡方多糖处理巨噬细胞48 h,光学显微镜下观察活细胞的形态,墨汁吞噬实验以及金黄色葡萄球菌吞噬实验观察巨噬细胞的吞噬能力,酶细胞化学染色观察巨噬细胞胞内酸性磷酸酶的变化。结果与对照组比较,(500、200、10)μg/m L调衡方多糖处理巨噬细胞后,巨噬细胞体积显著增大,对墨汁和金黄色葡萄球菌的吞噬能力均显著提高,酸性磷酸酶的活性也明显增强,并与多糖浓度呈正相关。结论调衡方多糖能增强巨噬细胞的吞噬功能,并提高细胞内酸性磷酸酶的活性。

Objective To study the immunoregulatory effect of Tiaohengfang polysaccharides（ THFPS） on the phagocytosis of macrophages. Methods According to the preparing method of Chinese medicine polysaccharides,crude polysaccharides were extracted from Tiaohengfang. Mouse peritoneal macrophages were separated and cultured. After the macrophages were treated with（ 500,200,10） μg / m L THFPS for 48 hours,the cell morphology was observed under a light microscope.Phagocytic tests of ink and Staphylococcus aureus were used to evaluate the phagocytosis of the macrophages,and cell enzyme chemical staining was applied to observe the changes of acid phosphatase in macrophages. Results Compared with the control group, the volume of macrophages significantly became bigger when they were treated with（ 500,200,10） μg / m L THFPS,the intake of ink and Staphylococcus aureus significantly increased,and the activity of acid phosphatase was also significantly enhanced,which was positively correlated with the dose of THFPS. Conclusion THFPS can enhance the phagocytosis of macrophages and increase the activity of intracellular acid phosphatase.