IL-1和IL-6的表达增强且与盘源性下腰痛改良日本骨科学会(mJOA)评分负相关

Increased IL-1 and IL-6 expressions are negatively correlated with modified Japanese Orthopedic Association(mJOA) scores of discogenic low back pain

目的检测白细胞介素1（IL-1）和IL-6在椎间盘源性下腰痛（DLBP）患者退变椎间盘中的表达,评估、记录其腰痛改良日本骨科学会（m JOA）评分,分析其相关性。方法选取同种族同地区30例45~65岁DLBP患者,另取10例以1年内无腰腿痛病史,因外伤导致腰椎骨折需行手术治疗,影像学资料未提示腰椎间盘退变作为对照。以腰痛m JOA评分评估DLBP的程度。免疫组织化学技术检测DLBP组与对照组椎间盘组织中IL-1和IL-6的表达,Western blot法检测椎间盘组织中IL-1和IL-6的含量,采用Pearson直线相关分析探讨IL-1、IL-6与m JOA评分之间的相关性。结果与对照组相比,DLBP组椎间盘组织IL-1、IL-6表达明显增强。Western blot实验证实DLBP组椎间盘组织IL-1、IL-6水平明显升高。DLBP组术前m JOA评分最低分7分,最高分16分。DLBP组IL-1、IL-6含量与m JOA评分显著负相关。结论 IL-1和IL-6在DLBP退变椎间盘含量增加,且与下腰痛m JOA评分呈负相关。

Objective To detect the expressions of IL-1 and IL-6 in the degenerative intervertebral discs of patients with discogenic low back pain（ DLBP）,record the modified Japanese Orthopedic Association（ m JOA） scores and analyze the correlation between the expressions and the scores. Methods The study chose 30 cases of DLBP aged between 45- 65 years old from the same race and district and 10 control cases of vertebral fractures scheduled for surgical treatment but with no history of low back pain within one year and no disc degeneration based on imaging data. The degree of DLBP was evaluated by m JOA scores. Immunohistochemistry and Western blotting were used to observe the expressions of IL-1 and IL-6 in the discs. The relationship between the IL-1,IL-6 expressions and the m JOA scores was revealed by Pearson linear correlation analysis. Results Compared with the control group,the expressions of IL-1 and IL-6 in the discs of DLBP patients increased significantly as immunohistochemistry and Western blotting indicated. The highest m JOA score of DLBP group was 16 points and the lowest was 7 points before operation. The expressions of IL-1 and IL-6 were negatively correlated with the m JOA scores of low back pain. Conclusion The expressions of IL-1 and IL-6 in the discs of DLBP patients were significantly elevated and negatively correlated with the m JOA scores of low back pain.