不同干预疗法对去卵巢骨质疏松大鼠肱骨骨矿物质含量影响

Effect of Different Treatment Methods on Bone Mineral Content in Tibia of Ovariectomized Osteoporosis Rats

目的:对比观察不同干预疗法对去卵巢骨质疏松大鼠肱骨骨矿物质含量的影响。方法:按体重将80只成年雌性SD大鼠分层后随机分为假手术组和去卵巢组。手术11周时,将去卵巢组大鼠按体重分层后又随机分为去卵巢组、跑台运动组、振动组、金雀异黄酮组、氯化锂组和雌激素组。跑台运动组每周进行4次45 min、速度18 m/min、跑道倾角5°的跑台训练;振动组每天进行2次15 min、频率90 HZ/min、7次/周的振动治疗;金雀异黄酮组每天按体重灌胃1次金雀异黄酮,剂量为1 mg/kg体重;氯化锂组每周按体重腹腔注射氯化锂3次,剂量为15 mg/kg;雌激素组每周按体重颈部皮下注射3次17β-雌二醇,剂量为25μg/kg。持续处理8周时,于末次处理结束36-48小时内,按解剖位置截取双肱骨,称量肱骨湿重、去脂肪干重以及煅烧后的灰重。结果:与假手术组比较,去卵巢组肱骨湿重/体重、去脂肪干重/体重和灰重/体重均显著下降;与去卵巢组比较,跑台运动组、振动组、金雀异黄酮组和雌激素组肱骨湿重/体重、去脂肪干重/体重、灰重/体重均显著增加,而氯化锂组虽有所升高,但差异无显著性。结论:除氯化锂处理外,其他几种处理均能减缓去卵巢骨质疏松大鼠肱骨骨量的丢失,对防治去卵巢骨质疏松大鼠的骨质疏松有一定的作用。

Objective： To compare the prevention mechanism of treadmill exercise, whole-body vertical vibration, genistein, and lithium chloride on postmenopausal osteoporosis. Methods： Eighty adult female SD rats were randomly divided into following two groups according to their body weight： sham-operation （Sham） group and ovariectomized （OVX） group. At 11 weeks of operation, the OVX rats were divided following five groups： the OVX treadmill exercise group （TE）, the whole-body vertical vibration group （WBVV）, the genistein group （G）, the lithium chloride group （LC）, and the estrogen group （E2）. The WBVV rats were placed on a vibration platform two days a day for 15 rain each time at 90Hz, 7 days a week. The G rats were taken genistein by intragastric administration at the dose of 1 mg/kg three times per week. The E2 rats were treated with estrogen at the dose of 25 p^g/kg by subcutaneous injection 3 times per week. The exercise rats were trained for 45 min at a speed of 18 m .min-~ with 5~ slope four times a week. After treated with regular exercise for 14 weeks, the tibia wet weight, the tibia fat-free dry weight, and the tibia ash weight was calculated by sensitive balance. Results： The ratio of wet weight to body weight, the ratio of fat-free dry weight to body weight and the ratio of ash weight to body weight in OVX group were significantly lower than that of in sham group. The ratio of wet weight to body weight, the ratio of fat free dry weight to body weight and the ratio of ash weight to body weight in TE group, WBVV group, G group, and E2 group were significantly higher than that of in OVX group. However, no significant difference was seen between LC group and OVX group Conclusion： Apart from LC treatment, the other four treatments could mitigate the mineral loss of humerus in OVX osteoporosis rats.