静磁场对小鼠黑色素瘤细胞B_(16)生长和氧化应激的影响

The Effects of Static Magnetic Fields on the Growth and Oxidative Stress of Mouse Melanoma Cells B_(16)

目的:利用小鼠黑色素瘤细胞B16,研究静磁场对肿瘤细胞生长和氧化应激的影响,探讨氧化应激介导静磁场影响肿瘤细胞生长的机制,为磁场在肿瘤疾病的治疗中的应用提供理论依据。方法:采用MTT法测定磁场对B16细胞活力的影响;利用流式细胞仪测定静磁场暴露对B16细胞周期分布的影响;利用生物化学方法测定磁场暴露对细胞氧化防御系统相关蛋白酶活性的影响。结果:24 h内50 m T-200 m T静磁场暴露可以抑制B16生长,但超过24 h的磁场暴露可以促进B16生长;100 m T和200 m T静磁场暴露对B16的细胞周期分布没有影响;B16暴露于100 m T和200 m T静磁场48 h,GST活性和GSH/GSSG水平表现为先上升后下降,SOD活性和T-AOC水平先下降后上升,CAT活性没有受到影响。结论:50 m T-200 m T静磁场可以抑制小鼠黑色素瘤细胞B16的生长,诱导肿瘤细胞产生氧化应激。

Objective： The purpose of this research is to investigate the effects of exposing to static magnetic fields（SMFs） on the growth and oxidative stress of mouse melanoma cells Bl6, and explore the relationship between oxidative stress and the tumor cell growth, and finally provide a theoretical basis for the application of SMFs in the treatment of tumor disease. Methods： The effects of SMFs on cells viability were examined by methyl-thiazol-tetrazolium（MTT）; the effects of SMFs on cell cycle distribution were detected by flow cytometry; the effects of SMFs on enzymes activity and antioxidant levels related to antioxidant defense system were monitored by biochemical methods. Results： Less than 24 hours exposing to SMFs（50 m T-200 m T） could inhibit the growth of B16, but more than24 hours exposing to SMFs（50 m T-200 m T） could promote the growth of B16; there’s no effects on cell cycle distribution after exposing to SMFs（100 m T, 200 m T）. During 48 hours SMF（100 m T, 200 m T） exposure, a rising trend displayed in the GST activity and the GSH/GSSG level, and then following a downward trend, at the same time the SOD activity and the total antioxidant capacity displayed a downward trend and then following a rising trend, and the CAT activity did not change. Conclusions： SMFs（50 m T-200 m T） could inhibit the growth of B16 cells, and induce oxidative pressure in the B16 cells.