肝性脑病患者脑脊液Tau蛋白水平测定及其临床意义

Level of Tau Proteins in CSF of Patients with Hepatic Encephalopathy

目的:探讨肝性脑病患者脑脊液中总Tau蛋白(t-Tau)和磷酸化Tau蛋白(p-Tau)水平的变化及其与疾病严重程度的相关性。方法:采集26例肝性脑病患者及31例健康对照的脑脊液标本,采用酶联免疫吸附法(Elisa)检测脑脊液中的t-Tau和p-Tau水平,分析其与Child-Pugh评分和West Haven分级的相关性。结果:(1)肝性脑病患者脑脊液t-Tau和p-Tau水平显著高于健康对照组(P<0.01)。(2)肝性脑病患者脑脊液t-Tau(γ=0.876,P<0.01;γ=0.952,P<0.01)、p-Tau(γ=0.808,P<0.01;γ=0.808,P<0.01)水平与Child-Pugh评分及West-Haven分级呈正相关。结论:由于脑脊液t-Tau和p-Tau水平为神经元损害的标志物,同时反应脑内应激状况,本研究证实肝性脑病患者脑内处于应激状态并有神经元损伤。

Objective： To investigate the levels of total Tau（t-Tau） and phosphorylated Tau（p-Tau） in the CSF of patents with hepatic encephalopathy（HE） and to investigate their correlations with the severity of the disease. Methods： Enzyme-linked immuno sorbent assay（Elisa） was used to investigate the CSF levels of total Tau, Tau181, Tau231 in 32 HE patients and 27 normal controls（NC）.Results： The CSF levels of t-Tau and p--Tau were significantly increased in HE patients when compared with normal controls（P〈0.01）.The CSF levels of t-Tau and p-Tau were positively correlated with the Child-Pugh Scores and West Haven Yale of the disease. Conclusions： As the levels of Tau proteins in CSF reflect the status of neuronal degeneration and the status of stress, the increased levels of Tau proteins in CSF indicate that HE patients experience significant neuronal degenerations and stress.