子痫前期患者血清对脐静脉内皮细胞CD40和NF-κB表达的影响

Influence of Preeclampsia Patients' Serum on Human Umbilical Vein Endothelial Cell CD40 and NF-κB Expression

目的:研究CD40和NF-κB在子痫前期患者组的脐静脉内皮细胞(HUVEC)中的表达,探讨其在子痫前期发病过程中的作用。方法:应用子痫前期患者组的血清冲击人HUVEC株,电镜下观察HUVEC的形态变化,流式细胞仪下测定细胞的凋亡,用RT-PCR方法检测HUVEC中CD40和NF-κB的表达情况。结果:1电镜下正常孕妇组HUVEC呈"铺路石"状单层镶嵌排列,子痫前期组HUVEC形态呈典型损伤改变,细胞稀疏,细胞核与细胞浆分界模糊不清,胞浆中有暗色颗粒。2流式细胞仪测定子痫前期组HUVEC的细胞凋亡较正常组明显升高,差异有显著意义(P<0.05);3 RT-PCR测定显示子痫前期患者组HUVEC中CD40m RNA及NF-Bm RNA水平均较正常组明显升高,差异有显著意义(P<0.05);4子痫前期组及对照组的CD40和NF-k B表达均呈显著性正相关。结论:CD40和NF-κB的激活均参与了子痫前期的发病机制,并在其中发挥重要作用。

Objective： To study expression of CD40 and NF-κB in preeclampsia patients’ human umbilical vein endothelial cell（HUVEC） and discuss its effect in the process of preeclampsia attack. Methods： Preeclampsia patients’ serum was used to impact people’s HUVEC strains, electron microscopy was applied to observe changes in HUVEC, flow cytometry was utilized to measurecell apoptosis,RT-PCR was adopted to detect expression of CD40 and NF-κB in HUVEC. Results： 1Under the electron microscopy, cells in normal pregnant women group were in single mosaic array in the formation of ＂paved road stone＂, but the preeclampsia group’s HUVEC cellular morphology had typical damage changes, cells were sparse, the boundary between cytoplasm and cell nucleus was unclear and dark color particles existed in cytoplasm; 2The preeclampsia group’s HUVEC cell apoptosis was obviously improved compared with the normal group and difference had obvious significance P〈0.05; 3RT-PCR showed that level of CD40 m RNA and NF-κBm RNA in preeclampsia group’s HUVEC was obviously improved compared with the normal group and difference had obvious significance P〈0.05; 4 CD40 and NF-κB expression of the preeclampsia group and the control group had significant positive correlation. Conclusion： Activation of CD40 and NF-κB took part in pathogenesis of preeclampsia and played an important role in it.