MMP1在炎症大鼠牙髓组织中表达

Expression of MMP1 in Rat Pulpitis

目的:探讨基质蛋白酶MMP-1在炎症性牙髓中的表达。方法:内毒素制备大鼠牙髓炎模型,取牙髓炎大鼠及健康大鼠牙髓组织行HE染色检测牙髓组织的组织形态学变化,MMP-1特异性免疫组织化学染色和Western blot等检测MMP-1在牙髓组织的表达,以明确MMP-1在大鼠急性牙髓炎期的空间分布改变及蛋白表达量变化。结果:大鼠牙髓组织急性炎症期牙髓炎模型中可见大量炎性细胞浸润,结缔组织有断裂破坏。免疫组织化学分析及Western Blot分析均显示牙髓炎组织MMP-1蛋白显著增高(P<0.05)。结论:MMP-1在牙髓炎组织中高表达,可能参与调节牙髓炎症反应的疼痛机制调节。

Objective： To investigate the expression of matrix metalloproteinases MMP-1 in rat pulp tissues and its role in inflammatory pain of pulpitis. Methods： LPS was used to construct rat pulpitis model. Hematoxylin-eosin staining was carried out to observe the morphological changes in pulp tissues. Then the expression of MMP-1 protein in dental pulp of both pulpitis group and normal control group were tested by immunohistochemistry and Western blot analysis. Results： Inflammatory cell infiltration was found in rat model of pulpitis, and the connective tissues had disruption. Immunohistochemistry and Western blot analysis showed that MMP-1 protein level was significantly higher in pulpitis group than that of control group（P〈0.05）. Conclusions： MMP-1 overexpression in pulpitis may have an important role in the pain mechanism of inflammatory response to pulpitis.