摘要
采用金黄色葡萄球菌肠毒素A（SEA）免疫Balb/c小鼠,制备相应的单克隆抗体（m Ab）,并以此为基础建立SEA胶体金检测方法,通过胶体金读数仪进行牛乳样品中SEA的定量分析。SEA胶体金试纸方法检测限为0.339μg/L,定量限为0.844μg/L,线性范围为1~16μg/L,相关系数R2=0.9933。采用配对t-检验分析,胶体金检测法与ELISA法无显著差异。
The immunochromatographic strip was developed using the anti-SEA monoclonal antibody（m Ab） produced. The strip was used together with the strip reader for the quantitative detection of DON in dairy milk samples. The results of the SEA colloidal gold test strip method showed that the detection limit of 0.339μg/L with quantitative limit of 0.844μg/L, the linearity range was 1~16μg/L and the correlation coefficient r2=0.9933. The pared t-test of colloidal gold test method and ELISA method showed no significant difference.
出处
《中国奶牛》
2016年第2期18-21,共4页
China Dairy Cattle
基金
北京市科学技术委员会资助(编号:Z131100004013014)
关键词
金黄色葡萄球菌
肠毒素
胶体金
SEA
单克隆抗体
定量检测
Staphylococcus aureus（SA）
Enterotoxin
ELISA
SEA
Monoclonal antibody
Colloidal gold
quantitative test
