幽门螺杆菌感染对血清及胃组织核组蛋白2/nesfatin-1表达的影响

Impact of Helicobacter pylori infection on serum and gastric tissue nucleobindin 2/nesfatin-1 levels

目的:研究幽门螺杆菌(Helicobacter pylori,H.pylori)感染对人血清及胃组织核组蛋白2(nucleobindin 2,NUCB2)/nesfatin-1表达的影响.方法:收集行胃镜检查的83例无症状体检者的空腹血清及胃窦黏膜,经13C呼气试验、快速尿素酶实验及组织切片Warth-Starry银染色三者共同确认分为H.pylori阳性组与H.pylori阴性组,采用酶联免疫吸附法及实时荧光定量PCR法分别测定血清NUCB2/nesfatin-1蛋白浓度及胃黏膜NUCB2 m RNA的表达量.结果:血清NUCB2/nesfatin-1蛋白浓度在H.pylori阳性组与H.pylori阴性组无明显差异(2.267 ng/m L±0.201 ng/m L vs 2.298 ng/m L±0.275 ng/m L,P>0.05);胃黏膜NUCB2 m RNA相对表达量在H.pylori阳性组明显高于H.pylori阴性组(1.336±0.324 vs 0.914±0.171,P<0.01).结论:H.pylori感染可导致胃黏膜NUCB2m R N A表达上调,但不影响感染者血清NUCB2/nesfatin-1浓度.

AIM： To investigate the impact of Helicobacter pylori（H. pylori） infection on serum and gastric tissue levels of nucleobindin2（NUCB2）/nesfatin-1 in humans.METHODS： Serum and mucosal samples were collected from 83 asymptomatic volunteers who received gastroscopy and had H. pylori infection confirmed by 13 C breath test, rapid urease test and Warth-Starry staining. The subjects were divided into an H. pylori positive group and an H. pylori negative group. Serum NUCB2/nesfatin-1 concentration was measured by enzyme-linked immunosorbent assay（ELISA） and the expression of NUCB2 m RNA in gastric mucosa was detected by real-time quantitative polymerase chain reaction.RESULTS：Serum NUCB2/nesfatin-1 concentrations in the H. pylori positive and negative groups did not differ significantly（2.298 ng/m L ± 0.275 ng/m L vs 2.267 ng/m L ± 0.201 ng/m L, P〈0.05）. The expression level of NUCB2 m RNA in the H. pylori positive group was significantly higher than that in the H. pylori negative group（1.336 ± 0.324 vs 0.914 ± 0.171, P〈0.01）. CONCLUSION： H. pylori infection induces the upregulation of NUCB2 m RNA in the gastric mucosa, but has no effect on serum NUCB2/nesfatin-1 concentration.