检测结肠癌根治性切除术前后胰岛素抵抗指数以及NF-κB、IL-6、TNF-α的表达

Detection of insulin resistance index and expression of NF- κB,IL- 6 and TNF- α in patients with colon cancer before and after radical resection

目的检测结肠癌根治性切除术前后胰岛素抵抗指数以及NF-κB、IL-6、TNF-α的表达情况,初步探讨NF-κB细胞因子在结肠癌术后胰岛素抵抗的作用机制。方法比较67例结肠癌根治性切除术患者手术前后胰岛素抵抗指数的变化,根据胰岛素抵抗指数增加值将手术患者分为低胰岛素抵抗组和高胰岛素抵抗组。分析各组术后NF-κB、IL-6和TNF-α的表达。结果术后血糖、胰岛素浓度及IR指数较术前明显升高,差异有统计学意义(P<0.01)。低IR组和高IR组术后IR指数较术前均显著升高,差异有统计学意义(P<0.01)。高IR组术后NF-κB、IL-6、TNF-α表达水平均高于低IR组,差异均有统计学意义(P<0.01)。术后NF-κB表达量与术后IR指数增加值呈正相关(r=0.596,P<0.01)。结论 NF-κB可能通过调节IL-6、TNF-α等炎症因子,进而影响在结肠癌术后胰岛素抵抗作用。

Objective To detect the expression of NF- κB,IL- 6,TNF- α and insulin resistance index in colon carcinoma before and after radical resection. And to investigate the mechanism of NF- κB cytokines in insulin resistance before and after colon cancer surgery. Methods Compare the changes of insulin resistance index in 67 cases of colon carcinoma after radical resection. And the patients were divided into low insulin resistance group and high insulin resistance group according to the increase value of insulin resistance index. Analyze the the expression level of NF- κB,IL- 6 and TNF- α of different groups after the operation. Results The postoperative blood glucose,insulin concentration and IR index were significantly higher than preoperative values,and the difference was statistically significant（ P〈 0. 01）. The postoperative IR index in both low IR group and high IR group were significantly higher than preoperative values,and the difference was statistically significant（ P〈 0. 01）.The expression levels of NF- κB,IL- 6 and TNF- α in the high IR group were significantly higher than those in the low IR group,and the difference was statistically significant（ P 〈0. 01）. The expression of NF- κB was positively correlated with the increase value of IR index after operation（ r = 0. 596,P〈 0. 01）. Conclusion NF- κB may influence the insulin resistance after colon cancer surgery by regulating inflammatory factors IL- 6 and TNF- α.