贵阳市市售水产品中副溶血性弧菌的污染状况和耐药性研究

Research on the contamination status of Vibrio parahaemolyticus in aquatic products from Guiyang markets and their antimicrobial resistance

目的了解贵阳市市售水产品中副溶血性弧菌污染状况,进一步对毒力基因和药敏进行检测,为防治副溶血性弧菌引起的食源性疾病提供依据。方法随机采集市售水产品149份,用PCR方法对分离出的副溶血性弧菌进行tdh、trh基因扩增,并进行药敏试验。结果 149份水产品中有28份样品分离出副溶血性弧菌;28株菌检出1株tdh阳性,所有菌株trh阴性;19株菌对氨苄西林耐药,耐药率为67.86%,有1株菌出现多重耐药,所有菌株对氨苄西林/舒巴坦、哌拉西林、哌拉西林/他唑巴坦、头孢替坦、头孢他啶、头孢曲松、头孢吡肟、氨曲南、亚胺培南、美罗培南、阿米卡星、庆大霉素、妥布霉素、环丙沙星、左旋氧氟沙星、呋喃妥因、复方新诺明敏感。结论贵阳市售水产品副溶血性弧菌污染已达一定程度,同时存在耐药情况,应加强水产品卫生监督及抗生素使用管理。

Objective To understand the contamination status of Vibrio parahaemolyticus（ Vp） in aquatic products from Guiyang markets,test the toxic genes and antimicrobial sensitivity of the isolated strains,so as to provide evidence for the control of food- borne diseases caused by Vp. Methods A total of 149 aquatic products were randomly collected from markets. Vp isolated in aquatic products were conducted for the amplification of tdh and trh by PCR method and for the drug resistance test. Results 28 samples were identified to contain Vp in 149 samples. All the 28 strains of Vp contained no trh genes,but one of them with tdh positive. 19 trains were resistant to ampicillin with the resistance rate of 67. 86%,and 1 strain was multi- resistant. All the strains showed sensitivity to ampicillin / sulbactam,piperacillin,piperacillin / tazobactam,cefotetan,ceftriaxone,cefepime,aztreonam,imipenem,meropenem,amikacin,gentamycin,tobramycin,ciprofloxacin,ofloxacin,nitrofurantoin and compound sulfamethoxazole. Conclusion The aquatic products were partly contaminated by Vp,and the isolated strains showed multiple antimicrobial resistance. Thus,it is necessary to strengthen hygiene supervision of aquatic products and the management of antibiotics＇ application.