高压氧对创伤性休克SD大鼠生存率的影响

Effect of Hyperbaric Oxygen on Survival Rate of SD Rats with Traumatic Shock

目的探讨高压氧对创伤性休克SD大鼠生存率的影响。方法水合氯醛麻醉SD大鼠后,利用2.5kg砖块从20cm高处垂直落下砸中左侧大腿中上1/3处,并右颈动脉插管放血,使得SD大鼠血压保持在40mmHg以上,以构建创伤性休克动物模型,而后将创伤性休克SD大鼠进行高压氧治疗60min,24h后采血,自动生化仪检测SD大鼠肝肾功能和存活率。结果实验结果显示成功构建SD大鼠创伤性休克动物模型;同时生化指标检测显示高压氧能够显著降低治疗组血清中各生化指标(P<0.05,n=24)和TNF-α(P<0.001,n=24)的含量,也可降低肝肾组织中MDA含量、提高SOD含量(P<0.05,n=24),可知高压氧处理能显著改善肝肾功能,使得SD大鼠存活率由70.83%提高到81.82%。结论高压氧能够通过保护创伤性休克SD大鼠的肝肾功能,提高创伤性休克过程中SD大鼠的存活率,这将为临床治疗创伤性休克患者提供重要的理论依据,为战争环境中抢救士兵提供重要的理论参考。

Objective To investigate the effect of hyperbaric oxygen on the survival rate of with traumatic shock CD.Methods Chloral hydrate-anesthetized rats were subjected to trauma at1/3of left thigh,which caused by vertical drop of brick（2.5kg）from a height of 20 cm.Furthermore,right carotid artery intubation was performed for bloodletting to maintain blood pressure higher than 40 mmHg.Then,rats with traumatic shock were treated with hyperbaric oxygen for60 min.Blood samples were collected 24 hafter treatment,and hepatic and renal function was determined using an automatic biochemical analyzer.In addition,the survival rate was recorded.Results The rat model of traumatic shock was established successfully.Hyperbaric oxygen treatment reduced serum contents of biochemical indicators and tumor necrosis factor-α（P〈0.05 and P〈0.001,respectively）.Moreover,hyperbaric oxygen treatment decreased malondialdehyde levels and increased superoxide dismutase level in the liver and kidney（P〈0.05）.In addition,the survival rate of rats with traumatic shock was increased from 70.83% to 81.82% by hyperbaric oxygen-induced improvement in hepatic and renal function.Conclusion Hyperbaric oxygen can improve the survival rate of SD rats with traumatic shock through protecting hepatic and renal function,which will provide an important theoretical basis for clinical treatment of patients with traumatic shock and an important reference for rescuing soldiers in the war environment.