摘要
目的探讨出生前邻苯二甲酸二（2-乙基己基）酯（DEHP）暴露对成年后仔鼠生殖腺凋亡相关基因表达水平的影响。方法将32只健康SD孕鼠随机分为对照（玉米油）组和2、10、50 mg/kg DEHP染毒组,每组8只。于妊娠第14~19天,采用灌胃方式进行染毒,染毒容量为10 ml/kg,每天1次。仔鼠出生后正常饲养10周,采用实时荧光定量PCR检测睾丸、卵巢组织内B淋巴细胞瘤基因-2（Bcl-2）及Bcl-2相关蛋白（Bax）的RNA表达水平。结果仅10、50 mg/kg DEHP染毒组成年雄性仔鼠睾丸组织内Bcl-2/Bax比值下降,差异均有统计学意义（P〈0.05）;而各剂量DEHP染毒组成年雄性仔鼠睾丸组织内Bcl-2、Bax基因表达水平均无明显改变（P〉0.05）。各剂量DEHP染毒组成年雌性仔鼠卵巢组织中Bcl-2、Bax基因的表达水平以及Bcl-2/Bax值与对照组相比,差异均无统计学意义（P〉0.05）。结论出生前暴露DEHP可能通过改变成年后雄性仔鼠睾丸生殖细胞凋亡相关基因Bcl-2/Bax比值,诱导生殖细胞凋亡,该途径可能是其雄性生殖毒性的机制之一。
Objective To investigate the effect of prenatal DEHP exposure on the expression of apoptosis-related genes in reproductive glands of adult offspring rats. Methods A total of 32 healthy SPF pregnant SD rats were randomly divided into corn oil,2,10 and 50 mg/kg body weight DEHP exposure groups,eight in each. The pregnant rats were treated with DEHP of different doses through gavage from gestation day 14（G14） to G19, once a day. The offspring rats were normally fed for ten weeks. The real-time quantitative PCR was used to detect the expression levels of apoptosis-related genes B-cell lymphoma-2（Bcl-2） and Bcl-2 associated X protein（Bax） in the testes and ovarian tissue. Results The ratio of Bcl-2/Bax in 10 and 50mg/kg DEHP exposure groups decreased significantly compared with the control group in testes（P〈0.05）. There was no significant difference in the expression levels of Bcl-2,Bax and the ratio of Bcl-2/Bax in ovarian tissue among the groups（P〈0.05）. Conclusion Prenatal exposure to DEHP may induce the decrease of the ratio of Bcl-2/Bax of testis in adult offspring rats,which may be one of the mechanisms underlying reproductive toxicity in males.
出处
《环境与健康杂志》
CAS
北大核心
2015年第11期945-948,共4页
Journal of Environment and Health
基金
国家自然科学基金(81273027)
