幽门螺旋杆菌诱发肝硬化大鼠高氨血症的实验研究

A Study on the Hyperammonemia in Helicobacter pylori induced Liver Cirrhosis in Rats

目的:探讨幽门螺旋杆菌与肝硬化大鼠高氨血症的相关性。方法:选取32只SD大鼠,将其随机分为正常组(n=8)、Hp感染组(n=8)、肝硬化组(n=8)、肝硬化合并Hp感染组(n=8)。肝硬化组及肝硬化合并Hp感染组给予50%四氯化碳橄榄油腹腔注射;正常组与Hp感染组给予同量的橄榄油腹腔注射。从建立模型的第9周开始,Hp感染组与肝硬化合并Hp感染组每周感染2次Hp,共8次。记录各组大鼠的一般状态,检测大鼠血氨水平及胃粘膜Hp数量。结果:与正常组比较,肝硬化组及肝硬化合并Hp感染组体重较低(P<0.05);与肝硬化组比较,肝硬化合并Hp感染组体重较低(P<0.05)。与正常组比较,其余各组胃粘膜Hp数量较高(P<0.05),与肝硬化组与Hp感染组比较,肝硬化合并Hp感染组胃粘膜Hp数量高(P<0.05)。与正常组比较,肝硬化组及肝硬化合并Hp感染组血氨水平高(P<0.05),与肝硬化组比较,肝硬化合并Hp感染组血氨水平高(P<0.05)。结论:幽门螺旋杆菌能诱发肝硬化大鼠高氨血症。

Objective： To investigate the hyperammonemia in Helicobacterpylon induced liver cirrhosis in rats. Methods： 32 experimental rats were selected and randomly divided into the normal group Hp infection group, cirrhosis group and cirrhosis complicated with Hp infection group, with eight rats in each group. The SD rats in the = cirrhosis group and cirrhosis complicated with Hp infection group were treated with 50% intraperitoneal injection of carbon tetrachloride, and the SD rats in the the normal group and Hp infection group were treated with the same amount of intraperitoneal injection of olive oil. The Hp infection group and cirrhosis complicated with Hp infection group were get 2 times a week Hp a total of 8 times. The general state, blood ammonia level and Hp of gastric mucosa were compared and recorded. Results： Compared with the normal group, the weight were lower in cirrhosis group and cirrhosis complicated with Hp infection group （P〈0.05）. Compared with the normal group, the Hp number of gastric mucosa were higher in others （P〈0.05）. Compared with the Hp infection group and cirrhosis group, the Hp number of gastric mucosa were higher in irrhosis complicated with Hp infection group （P〈0.05）. Compared with the normal group, the blood ammonia level were higher in cirrhosis group and cirrhosis complicated with Hp infection group （P〈0.05）. Compared with the cirrhosis group, the blood ammonia level were higher in cirrhosis complicated with Hp infection group （P〈0.05）. Conclusion： Helicobacter pylori could induce hyperammonemia in liver cirrhotic rats.