ANP在大鼠高血压进展为心力衰竭中的作用

The Effect of ANP in High Blood Pressure Progression to Heart Failure in Rats

目的:探讨心房钠尿钛ANP在高血压心力衰竭发展过程中的变化和氨氯地平保护心肌细胞的作用。方法:对大鼠行腹主动脉结扎术,术后随机选择40只大鼠分为氨氯地平(中、高、低剂量)组以及模型组,另外选取10只健康雄性SD大鼠作为假手术组。采用ELISA方法检测各组血清ANP浓度变化。结果:随着心功能不全加重,ANP水平逐渐上升。氨氯地平用药组大鼠的心功能改善明显优于模型组,ANP明显降低,且随着氨氯地平用药量上升,心功能不断改善,ANP显著下降,P<0.05。结论:对血清ANP浓度进行测定能够反映出高血压大鼠模型心室功能不全及充血性心力衰竭严重程度。而氨氯地平能够影响机体的ANP分泌对心肌细胞起到保护作用,从而有效抑制心室重构,延缓高血压心力衰竭疾病进展。

Objective： To study the effect of ANP on high blood pressure heart failure progression in rats and myocardial cells protective effect of amlodipine. Methods： Divided 40 hypertensive rats which received abdominal aorta ligation into amlodipine （middle, high and low doses） group and model group, chose 10 healthy rats as sham-operated group. Using ELISA method to detect the serum concentration of ANP. Results： With cardiac insufficiency aggravating, ANP level was rising gradually. Cardiac function of amlodipine treatment rats was significantly better than the model group, and ANP decreased obviously. With amlodipine dose increasing, heart function improved and ANP dropped significantly, P 〈 0.05. Conclusion： The serum ANP level can reflect the ventricular dysfunction and congestive heart failure severity of hypertensive rats model. And amlodipine can affect the body＇s secretion of ANP and to protect myocardial cells, thus effectively inhibit ventricular remodeling, delay the development of high blood pressure and heart failure disease.