摘要
为了保持细胞的生物活性,基于皮克林乳液开发了条件温和的细胞包裹技术,成功地制备了壳聚糖/Fe3O4/聚(N-异丙基丙烯酰胺-co-丙烯酸)/大肠杆菌复合微囊。首先采用微波辅助分散聚合法和共沉淀法分别制备了聚(N-异丙基丙烯酰胺-co-丙烯酸)(P(NIPAM-co-AAc))微凝胶与油酸改性的Fe3O4纳米粒子,并用其作为稳定剂制备皮克林乳液。最后大肠杆菌(E.coli)加入水相后分散在乳液液滴内部,使用异硫氰酸荧光素标记的壳聚糖(FITC-CS)交联油水界面的Fe3O4与P(NIPAMAAc)微凝胶形成微囊。通过扫描电镜、透射电镜和激光共聚焦荧光显微镜(LCSM)对制备的凝胶、纳米粒子和复合微囊的形貌结构和组成进行了观察和分析。
In order to maintain cell biological activity,the cell encapsulation with wild condition was developed based on Pickering emulsion,the Chitosan/Fe3O4/poly（N-isopropylacrylamide-co-acrylic acid）/Escherichia coli composite microcapsule was prepared and characterized.The P（NIPAM-co-AAc）microgels and oleic acid-modified Fe3O4 nanoparticles were prepared by microwaveassisted dispersion polymerization and co-precipitation method,respectively,and then they were used as the stabilizer to fabricate Pickering emulsion.Chitosan was used to crosslink the P（NIPAM-co-AAc）microgels and Fe3O4 nanoparticles on the interface of the emulsion droplets which leads to the formation of novel organic/inorganic/biological composite microcapsule.Laser confocal scanning fluorescence microscopy（LCSM）was used to identify emulsion type of the Pickering emulsion.The morphology and structure of the composite microcapsule was observed by SEM and LCSM.
出处
《中国科技论文》
CAS
北大核心
2015年第24期2847-2849,共3页
China Sciencepaper
基金
高等学校博士学科点专项科研基金资助项目(20130121130006)
关键词
复合微囊
细胞包裹
大肠杆菌
composite microcapsules
cell encapsulation
Escherichia coli
