基于MAPK通路模型的电磁非热生物效应机制研究

Research on the mechanism of non-thermal electromagnetic bio-effects based on the model of MAPK pathway

针对极低频电磁场与细胞的能量耦合问题,采用化学动力学方程构建与电磁场敏感蛋白激酶ERK相关的MAPK信号通路模型。通过敏感度分析方法发现显著影响ERK活性的模型参数,并研究该参数影响ERK活性的规律。仿真实验结果表明,MAPK/ERK激酶的反应速率增大对ERK的活性激活过程和稳态分子数影响不大,但降低却会显著影响ERK的激活触发时间,且稳态分子数呈下降趋势。结合ERK活性的生物学意义表明,无论电磁场产生有害健康还是治疗疾病作用,均是通过降低MAPK/ERK激酶的生化反应速率而导致的后续生化反应结果。

For the problem of energy coupling between the extremely low frequency electromagnetic fields and cells,the MAPK signaling pathway models associated with electromagnetic sensitivity protein kinase ERK are built based on chemical kinetics equation.The model parameters that affect ERK activity significantly are found by sensitivity analysis and the law of ERK activity affected by this parameter is investigated.The simulation results show that there is little effect on the activity of ERK activation and steady-state molecular number if increasing the reaction rate of MAPK/ERK kinase.However,if decreasing the reaction rate of MAPK/ERK kinase the trigger time of ERK activation is significantly affected,and the steady-state molecular number decreases.Combining with biological significance of ERK activity,the work indicates that whether the electromagnetic fields cause physically damaging or disease treatment is due to the result of decreasing the reaction rate of MAPK/ERK kinase.