白芍多糖中单糖的组成分析

Analysis of Monosaccharide Compositions in Paeoniae Radix Alba Polysaccharide

目的:分析不同生长年限白芍及不同组分白芍多糖中多糖的单糖组成及摩尔比。方法:建立PMP柱前衍生化HPLC法测定不同生长年限的白芍以及采用分级醇沉法得到的白芍多糖不同组分中多糖的单糖组成和摩尔比。结果:白芍多糖中的单糖主要是由甘露糖、鼠李糖、葡萄糖、阿拉伯糖、半乳糖及木糖组成,摩尔比为:156.26∶0.73∶0.85∶1.03∶1.17∶0.79。随着生长年限的增加,白芍多糖中葡萄糖摩尔百分比逐渐增加,其余5种单糖摩尔百分比逐渐降低;白芍多糖不同组分中葡萄糖的含量随着多糖分子量的减小,葡萄糖摩尔百分比逐渐增加,其余5种单糖摩尔百分比逐渐降低。结论:PMP柱前衍生化HPLC法可用于白芍多糖中单糖组成分析,不同生长年限白芍及不同组分白芍多糖中单糖摩尔比不同,葡萄糖是构成白芍多糖的主要单糖。

Objective： To analyze the monosaccharide composition and the molar ratio of Paeoniae Radix Alba polysaccharide（ PYPS）. Methods： Pre-column derivation HPLC method was developed to determine the monosaccharide composition and the molar ratio of PYPS from Paeonia lactiflora root of different growth years and obtained by graded alcohol precipitation. Results： The results of HPLC analysis showed that PYPS consisted of Man,Rha,Glc,Ara,Gal and Xyl with the molar ratio of 156. 26∶ 0. 73∶ 0. 85∶ 1. 03∶ 1. 17 ∶ 0. 79.Glc molar ratio increased gradually with the increasing of growth years,while the other five monosaccharides decreased. Conclusion： Precolumn derivation HPLC method can be used to determine the monosaccharides in PYPS,molar ratio are differently in the different growth years and molecular weight of the PYPS,Glc is the main monosaccharide of PYPS.