中药水提物对痤疮丙酸杆菌的体外抑制作用

Antimicrobial activities of traditional Chinese medicine aqueous extract on propionibacterium acnes in vitro

目的 探讨中药水提物对痤疮丙酸杆菌标准株及痤疮丙酸杆菌临床株的体外抑制作用。方法采集痤疮患者皮损内容物，35℃厌氧培养48h，采用系列生化实验及VITEK全自动微生物鉴定仪鉴定为痤疮丙酸杆菌；以琼脂稀释法分别检测19种中药水提物对痤疮丙酸杆菌的体外抑菌作用。结果黄柏、黄芩、大黄的最小抑菌浓度（MIC）为25mg／ml；金银花、苦参的MIC为50mg／ml；连翘的MIC为100mg／ml；地肤子、白头翁、百花蛇舌草、鱼腥草、马齿苋、旱莲草、紫草、板蓝根、大青叶、蒲公英、车前子、白芷、蛇床子等13味中药在最高浓度200mg／mt进行测试时仍未产生抑菌作用。结论黄柏、黄芩、大黄、金银花、连翘、苦参等6种中药水提物对痤疮丙酸杆菌具有良好的体外抑制作用。

Objective To observe the effects of 19 kinds of traditional Chinese medicine （TCM） aqueous extracts in vitro on the standard and clinical strains of propionibacterium aches. Methods We collected lesion contents of acne vulgaris patients and conducted anaerobic cultivation of propionibacterium aches at 35℃ for forty-eight hours. After tested by oxygen resistance experiment, Gram staining microscopy, catalytic test, nitrate test and sugar fermentation experiment, ANI anaerobic identification card, and VITEK fully-automatic microbe instrument, the strains were identified as propionibacterium acnes. We used AGAR dilution method to test the MIC values of various TCM aqueous extracts. Results The MIC values of Cortex phellodendri, Scutellaria baicalensis, and Rhiubarb were 25 mg/ml, respectively; the MIC values of Sophora flavescens and Honeysuckle were 50 mg/ml; the MIC values of Forsythia was 100 mg/ml. 13 kinds of TCM aqueous extract did not produce bacteriostasis at the highest concentration of 200 mg/ml including Belvedere fruit, Chinese bulbul, Hedyotis diffusa, Houttuynia cordata, Purslane, Yerbadetajo herb, Radix isatidis, Lithospermum erythrorhizon, Folium isatidis, Taraxacum, Semen plantaginis, Angelica dahurica, and Fructus cnidii. Conclusions Aqueous extracts of Cortex phellodendri, Scutellaria baicalensis, Rhubarb, Sophora flavescens, Honeysuckle and Forsythia have good inhibitory effects in vitro on Propionibacterium aches.