Caco-2细胞研究石榴皮中活性成分鞣花酸的摄取特性

Uptake determination of Ellagic acid in Caco-2 cells

目的：研究鞣花酸在Caco-2细胞的摄取特性。方法：采用Caco-2细胞单层模型研究鞣花酸在不同给药时间、药物浓度、p H介质、培养温度、有无抑制剂条件下对细胞摄取的影响。采用高效液相色谱法（HPLC）测定鞣花酸在细胞中的含量。结果：鞣花酸在0.0254~1.016μg范围内呈现良好的线性关系（r=0.9997）。鞣花酸在Caco-2细胞上的摄取与给药时间、药物浓度、p H介质、培养温度、抑制剂密切相关。结论：建立的HPLC检测方法简便、灵敏、准确,鞣花酸在Caco-2细胞上的摄取主要是主动转运,抑制剂不同程度参与鞣花酸的转运过程。

Objective：To study the uptake status of Ellagic acid in Caco-2 cells.Method：Optimization using Caco-2 cell monolayer model research Ellagic acid in different time,concentration,p H,temperature,under the condition of P-gp inhibitors effects on cellular uptake.To establish high performance liquid chromatography（HPLC）determination of the content of Ellagic acid in cells.Result：The linearity of Ellagic acid concentrationcurve was found in the rang of 0.0254~1.016 μg（r=0.9997）.Uptake determination of Ellagic acid in Caco-2 Cells absorb a certain amount of time,concentration and p H dependence and Pgp inhibitors can significantly increase the Ellagic acid intake.Conclusion：This method is simple and sensitive HPLC assay can be applied to determine Ellagic acid concentrations in Caco-2 cells.Ellagic acid inhibitors in different level of transfer process.